Anti-oxidative Effect Of Soyas Aponin In Livers Of Aging Mice Induced By D-galactose

Objective:To Study the anti-oxidative effect of soyasaponin in liver of aging mice induced by D-galactose.Methods:Sixty kunming mice are randomly divided into six groups, namely the normal control, D-galactose model, as well as the low, middle, and high dose soyasaponin, and vitamin E as the positive control. Mice of the low, middle and high dose soyasaponin groups are given 25,50, 100mg/kg of soyasaponin through the gastric gavage once daily for seven weeks. Mice of vitamin E positive control group are given 50mg/kg vitamin E through the gavage once daily for seven weeks. Except for the normal control group, mice of other groups are injected with 300mg/kg D-galactose once daily through the experiment. After fasting 24 hours, the mice are decapitated, blood and liver tissue are taken for assays. The livers are detected with the HE staining and electron microscope detection, respectively. The blood 8-OHdG, and liver glutathione (GSH), glutathione oxidized (GSSG), glutathione peroxidase (GSH-PX) and catalase (CAT) are detected. The liver mitochondrial Na+-K+-ATPase, Ca+-Mg+-ATPase, SOD, MDA are tested. The protein expression of p38, p-p38, JNK, p-JNK, Akt and p-Akt are tested by western blot method.Results:1.The results showed that D-galastose caused the damage in the liver of aging mice, while soyasaponine has a protective effect on the hepatic damage of aging mice induced by D-galactose. Compared with the normal control group, TGSH, GSH contents and GSH-PX, CAT activities of D-galactose model group decreased (P<0.01); compared with D-galactose model group, TGSH, GSH contents and GSH-PX, CAT activities of soyasaponin groups significantly increased (P<0.05).3. Compared with the normal control group, the activities of Na+-K+-ATPase, Ca2+-Mg2+-ATPase and Mn-SOD in liver mitochondria of D-galactose model group decreased (P<0.05), while the content of MDA increased; compared with D-galactose model group, the activities of Na+-K+-ATPase, Ca2+-Mg2+-ATPase and Mn-SOD in liver mitochondria of soyasaponin groups significantly increased, and the content of MDA decreased (P<0.05).4. Compared with the normal control group, the protein expression of p-p38 MAPK, p-JNK, p-PI3K and p-Akt in D-galactose model group significantly increased; compared with D-galactose model group, protein expression of p-p38 MAPK, p-JNK, p-PI3K and p-Akt in soyasaponin groups decreased.Conclusion:Soyasaponin has a protective effect on the hepatic damage of aging mice induced by D-galactose, the mechanism may be related to the improved antioxidant capacity of the liver of mice.