Research On YAP Regulating The Neurite Axon Of N2a Cells

In the present study, YAP was proved to increase mi R-29 a, but the relevant molecular mechanism is not clear. Also, growing evidence indicates the mi R-29 a promotes the neurite outgrowth by decreasing PTEN. Results showed that mi R-29 a expression levels increased during transfecting YAP in N2 a,while PTEN decreased. In the meantime, a protein expression assay and RT-PCR presented the development of neurite outgrowth via GAP-43 and NF-200. The expression of mi R-29 a, PTEN GAP-43 and NF-200 showed an opposite tendency when YAP was down-regulated. By treating N2 a with mi R-29 a mimic and inhibitor, we also found the same results. In silico analysis of mi R-29 a, its promoter may have a binding site for YAP. Based on a luciferase reporter assay, we demonstrate that YAP could increase mi R-29 a level by targeting to the promoter of mi R-29 a. In conclusion, the results identify that YAP promote the neurite outgrowth via targeting mi R-29 a and that it may be an effective therapeutic medicine for neural disease.