Expression Of MicroRNA-29a Regulated By YES-associated Protein Modulates The Neurite Outgrowth In N2a Cells

Over the past years,the Hippo pathway has emerged as a key regulator of organ size.And in the present study,Yes-associated protein(YAP)isthe main downstream target of the mammalian Hippo pathway,promotes the organ growth.And our team has proved that YAP can promote the neurite outgrowth in N2 a cells,yet the underlying molecular mechanism of this regulation remains unclear.Previous research has proved that YAP overexpression increases the expression of miR-29 a,but the relevant molecular mechanism is not clear.Also,growing evidence indicates that the high-level miR-29 a promotes the neurite outgrowth by decreasing PTEN(Phosphatase and tensin homologue deleted on chromosome 10).We demonstrate that YAP downregulates PTEN by inducing miR-29 to inhibit PTEN translation.At last,our team show that PI(3)K-mTOR is a pathway modulated by YAP to regulate the neuriteoutgrowh in N2 a cells.Our studies reveal a functional link between Hippo and PI(3)K-mTOR,providing a molecular basis for the coordination of these two pathways in organ size regulation.In silico analysis of miR-29 a,its promoter may have a binding site for YAP.Based on a Chromatin immunoprecipitation(Ch IP)assay,we demonstrate that YAP could increase the mi R-29 a expression by targeting to the promoter of miR-29 a.In conclusion,the results identify that YAP promotes the neurite outgrowth via targeting to the promoter of miR-29 a,and it may be an effective therapeutic medicine for neural disease.