The Relationship Between The Proportion Of Treg/Th17 Cells In Peripheral Blood Of Patients With Unexplained Recurrent Spontaneous Abortion And Pregnancy Outcomes

Purpose: To investigate predictive value of Treg and Th17 cells in the peripheral blood of patients with unexplained recurrent spontaneous abortion(URSA)for their pregnancy outcomes.Method:The peripheral blood was collected from 60 URSA pregnant women and 56 normal healthy pregnant women during their first trimester. The counts of Treg cells, Th17 cells, CD4+T cells and total lymphocytes in the peripheral blood were measured by flow cytometry. Calculated the ratios of Treg/CD4+T cells, Treg/total lymphocytes, Th17/CD4+T cells, Th17/total lymphocytes and Treg/Th17, then the outcomes of their pregnancy were followed up. According to the different outcomes of pregnancy, URSA were divided into two groups: URSA successful delivery group and URSA miscarriage again group. Comparedthe ratios of Treg/CD4+ T cells, Treg/total lymphocytes, Th17/CD4+ T cells, Th17/total lymphocytes and Treg/Th17 of the URSA successful delivery group, URSA miscarriage again group and the normal successful delivery group respectively.Results:1.The ratios of Treg/CD4+T cells, Treg/total lymphocytes and Treg/Th17 in the URSA successful delivery group were significantly more than the URSA miscarriage again group, however, the ratios of Th17/CD4+T cells and Th17/total lymphocytes were less(P<0.05). 2. There were no significant difference emerged in the ratios of Treg/CD4+T cells, Treg/total lymphocytes, Th17/CD4+T cells, Th17/total lymphocytes and Treg/Th17 between the URSA successful delivery group and the control group(P >0.05). 3. The ratios of Treg/CD4+T cells, Treg/total lymphocytes and Treg/Th17 in the URSA miscarriage again group were significantlyless when compared to the control group, but the ratios of Th17/CD4+T cells and Th17/total lymphocytes were more(P <0.05).Conclusion:The counts of Treg and Th17 cells in the peripheral blood of unexplained recurrent spontaneous abortion(URSA) might be used to predict the outcomes of their pregnancy.