Fluorescence In Situ Hybridization Detection Cytogenetics The Relationship Between The Prognosis Of Patients Within MCL

Background:Mantle cell lymphoma is a typical set of non-hodgkin’s lymphoma, accounting for about 6% of non-hodgkin’s lymphoma patients. Mantle cell lymphoma as a kind of difficult to treat, rarely cure a disease. The median survival of about 3 years, but now the estimation new patients survival time with application of new detection methods, such as multiparameter flow cytometry analysis technology, fluorescence in situ hybridization (FISH) technology, molecular biology experimental technique of auxiliary diagnostic helping improve patient diagnose level, So make patients to extend the survival time about 6 years.Mantle cell lymphoma is a subtype of B cell lymphoma, because before the germinal center B cell, so the expression of cell surface antigen CD5, CD20 cell surface marker, such as the cells exist in normal follicular germinal center position. Set of lymphoma cells because of chromosome 11 and 14 translpcation, lead to excessive expression of cyclinD1 protein. Chromosome specific ectopic locations in t (11; 14) (q13, q32). Fluorescence in situ hybridization technology as a kind of cell genetics, use of fluorescent probe to detect patients’genetic manipulation.Objectives:Analysis by using the method of fluorescence in situ hybridization in patients with TP53 lack influence on the prognosis of patients with invasive mantle cell lymphoma, and study aggressive set of lack of P53 in mantle cell lymphoma patients at the same time, explore the biological characteristics of fluorescent in situ hybridization to detect other sites in the patient’s prognosis.Methods:Retrospective analysis of Blood Diseases Hospital, Chinese Academy of Medical Sciences in July 2003 to January 2015 of 50 cases with peripheral blood and bone marrow involvement between sets of data cell lymphoma patients.And the use of fluorescence in situ hybridization method to detect patients with D13S25/13 q14, ATM/ 11 q22, p53/17 p13, c-MYC/8 q24, BCL2/18 q21 and IGH/CCND1/1 (11,14), a total of 6 kinds of DNA probes, then through SPSS methods to analyze the influence of genetic correlation and prognosis.Results:The median age of the patients was 55.5 years old; males are 38 patients; 18 patients with B symptoms, with 36 cases (72%) patients in the symptoms associated with spleen to begin with. Media white blood cell (WBC) count was 44.73 (2.63193.78) x 109/L, the median beta 2 microglobulin was 4.45 (1.95 to 12.7 mg/L). Based on MCL international prognostic score (MIPI) system,26 cases (52%) patients in high-risk groups, moderate and low risk groups each have 12 cases (24%).Univariate analysis by SPSS, lack of P53’s relation with PFS and OS prognostic factors and the test site and found that the relationship between Del 13 q14, Del 17 P, MYC amplification or obtain all of PFS and OS statistically significant, P values of PFS are respectively 0.003,0.000,0.000, P values of OS were 0.012,0.000,0.000;Multivariate analysis of the relationship between various factors and PFS and OS found Del 17, MYC amplification or obtain the two factors on its independent prognostic significance, PFS related P values were 0.039,0.020, P values associated with the OS 0.045,0.026.Conclusion:Lack of P53 in mantle cell lymphoma patients as a common set of cell mutation, and relevant PFS and OS in the prognosis of patients with independent prognostic factors.