Research On The Effect Of Rho Kinase On Vascular Remodeling In OSAHS With Hypertension Model

Objective: To investigate the blood pressure level and condition of vascular remodeling in CIH model, and the effects of ROCK inhibitor-Y27632 on vascular remodeling in OSAHS. Methods : 24 adult male SD rats weighting 180~200 grams were divided into four groups randomly, which are normal oxygen(A) group, Y27632 control(B) group, CIH(C) group and CIH+Y27632(D) group. Each group contained six rats. Rats of two CIH groups were placed in the low oxygen cabin suffering intermittent hypoxia 8 hours a day for 10 weeks. Rats of two normal oxygen groups were placed in the same cabin without intermittent hypoxia. Each rat’s blood pressure was measured before and every two weeks during the experiment. Rats’ serum and abdominal aorta were obtained after the experiment. Enzyme linked immunosorbent assay(ELISA) was used to test SOD, MDA, hs-CRP and sE-s in serum. Western-blotting method was used to test the content of TGF-β1 and α-SMA in abdominal aorta. Hematoxylin-eosin staining and Masson staining were used to analyze vascular remodeling in abdominal aorta. Results: Compared with A and B two groups, the blood pressure of rats in C group was significantly increased, MDA and hs-CRP and s E-s in serum were significantly increased, level of SOD was decreased, the abdominal aortic pathological section showed vascular remodeling, TGF-β1 content increased significantly while α-SMA content decreased significantly(P<0.05); Compared with C group, the blood pressure of rats in D group was significantly decreased, the levels of MDA and hs-CRP and sE-s were significantly decreased, level of SOD increased significantly.(P<0.05); There was no statistical difference between A and B groups. Conclusions:(1) Oxidative stress caused by intermittent hypoxia is involved in the pathogenesis of OSAHS with hypertension.(2) Oxidative stress and inflammation may be important mechanisms of vascular remodeling in OSAHS-induced hypertension.(3) ROCK inhibitor Y27632 improves vascular remodeling in CIH model by reducing the inflammatory reaction and ameliorating the phenotype transformation of vascular smooth muscle cells mediated by TGF- beta 1.