Preparation And Characterization Of Cuttlebone/Racemic Polylactic Acid Composite Artificial Bone

Objective: with the increasing demand of the materials on clinical bone implant, developing the substituted materials of artificial bone, similar to natural bone, has become popular. In recent years, synthetic bone, a kind of bionic bone, has been widely applied in clinical medicine. And the biological of scaffold materials, pore size, porosity, biomechanical and degradation have a direct effect on bone transplantation. Cuttlebone, which is the shells of marine mollusk cuttlefish, has good biocompatibility and also has a lot of fine pore structures. Micropores communicate with each other, but they are brittle. The modification of cuttlebone by adding racemic polylactic acid, the independent research and development of cuttlebone / racemic polylactic synthetic acid composite artificial bone, and the detection of their various performance, lay the foundation for developing new bone materials.Methods :With the salting out method, we make the composite artificial bone from cuttlefish bone(CB) and racemic polylactic acid(PDLLA), respectively following the quality ratio as 1:2, 2:1, 1:1. After that, we detect and compare them with pure sepium(CB).The microstructure, porosity, water binding force and the biological mechanics, the properties are measured. Results and Conclusion: Pure sepium(CB) has spines shape, texture clear, cellular. Preparation of composite artificial bone is similar to cancellous bone, whose big pore diameter is about 500 ~ 1500 microns and pore diameter is about 150 ~ 300 microns. Composite artificial bone has less microporous and better connectivity between the holes. the porosity of pure sepium(CB) is around 85% ~ 90%, which is higher than that of three composite artificial bone(62%-67%)(P<0.05). Composite artificial bone water binding force, mechanical strength and degradability of the three composite artificial bone in vitro were superior to pure sepium(CB). There is no statistical significance between three composite artificial bone and the mechanical strength(P > 0.05). With the salting out method the different proportion of sepium(CB)/racemic polylactic acid(PDLLA),composite artificial bone has good microstructure, mechanical properties and degradation performance.