BMSCs-GFP Transplantation Research For The Treatment Of Traumatic Brain Injury In Mice

Background:In today’s society craniocerebral injury is next to disease of heart head blood-vessel,cancer serious harm to the health of the people of the third big disease, because of its high mortality and high morbidity, attaches great importance to by the people for many years.Nearly ten years, along with the progress of the surgery method and idea to read,and all kinds of advanced and effective treatment in the clinical application of severe head injury mortality had significantly lower, but significantly increased morbidity.Most of the patients with brain injury left behind by different degree of nerve function obstacle, such as cognitive and psychiatric disorders, aphasia, hemiplegia and even long-term plant survival state, etc.Nerve dysfunction caused by traumatic brain injury has become a big difficult problem faced in the process of brain injury treatment,because the traditional treatment cannot promote neuron regeneration, repair the damaged neurons.Therefore to seek a safe and effective way to repair due to traumatic brain injury on brain structure and function of injury has become a current neurosurgery is faced with an important research subject.Stem cells found in recent years, has brought people hope and faith, a large number of studies have shown that stem cell transplantation is the only ways to repair and replace necrotic brain cells, can obviously improve the function of damage that results when brain cells, shows a good prospect.Between Bone marrow Mesenchymal Stem Cells(ipads Mesenchymal Stem Cells, BMSCs) since it has sources, with autograft, allograft easily acquired, in vitro proliferation and multi-directional differentiation potential, genetic stability and low immunogenicity, become the hot topics in the study of Stem Cells to treat brain injury.But stem cells because of its many uncertain factors, for the treatment of traumatic brain injuries is still in the early stage in animal experiments.This research through the establishment of traumatic brain injury in mice model, after the ball the veintransplantation GFP marker of BMSC, observe the migration of BMSC around the focal trauma and engraftment, in order to investigate the effectiveness of mesenchymal stem cells in the treatment of craniocerebral injury and safety.Objective:Explore the ball after venous plexus transplantation of bone marrow mesenchymal stem cells(BMSCs) between the effectiveness and safety of treatment of traumatic brain injury in mice, and to explore its possible mechanism.Methods:By the methods of improved Feeney(free fall) build a traumatic brain injury in mice model, 90 mice were randomly divided into 3 groups: control group, model group and BMSCs.BMSCs after the success of the group in building 1 h after the ball the venous plexus injection GFP marker of BMSC, model group, amount of saline injection,control group only line and suture.Observe each group the following indicators: 1, to observe 6 h, 12 h, 24 h after injury, 3 d and 7 d different time points, such as nerve injury severity score(NSS);2, through the brain tissue slices TTC(2,3,5- three benzene tetrazolium chloride) dyeing calculate 3 d and 7 d after injury cerebral contusion area;3,2 h, 6 h, 24 h after injury and 3 d using the confocal fluorescence microscopy BMSCs after brain damage, GFP colonize the trauma of migration and affection;4, 3 d and 14 d after injury detection each wound area neuron specificity enolization enzyme(NSE) and glial fibers acidic protein(GFAP).Results:By using free fall blow successful build craniocerebral injury model in mice, a significant injury or after nerve dysfunction.BMSCs group since the 3 d brain contusion area is significantly reduced in the model group, 7 d after injury NSS scores significantly decreased, compared with model group differences were statistically significant(P < 0.05).BMSCs- GFP transplantation in brain contusion and the surrounding green fluorescence distribution, with the passage of time, the green fluorescence distribution quantity increase gradually, gradually after the mesenchymal stem cell transplantation between damage to the stove and the surrounding migrationengraftment.Results of immunohistochemical detection showed that the 3 d and 14 d after BMSCs group mice brain tissue damage area NSE, GFAP positive cells are increased significantly than control group, model group, 14 d after injury differences statistically significant(P < 0.05).Conclusion:Traumatic craniocerebral injury BMSC transplantation into the mice, that early after injury to the brain damage area migration and accumulation, and survival,proliferation and differentiation in the damage zone, to increase the number of neurons and glial cells, reduce the area of brain contusion, thus promoting the recovery of neurological function, within the brain tissue injury may be related to stem cells to improve the environment, promote new angiogenesis and secretion of nerve growth factor and other factors related.