Preparation Of Mineralized Collagen Coating On Titanium Implant Surface And Evaluation Of Its Biological Properties

Objective:Electrochemical deposition methods with different parameters could prepare mineralized collagen coatings with different spatial structures. This study attempt to analyse mineralized collagen coatings’ effects on osteoblast MC3T3-el,by observing its adhesion, proliferation and differentiation, and to explore more beneficial electrochemical parameters to osteogenesis.Method:The mineralized collagen coatings on titanium surfaces were prepared by electrochemical deposition method, and the surface morphology was observed by scanning electron microscope. In the mineralized collagen coating and the control groups, MC3T3-e1 osteoblasts were cultured on the surfaces. Analyse the adhesion and proliferation of osteoblasts by MTS detection at 24h and 72h. The morphology and adhesion of osteoblasts were observed by scanning electron microscope at 24h. Evaluate the status of osteoblasts’differentiation by detecting ALP, COL-1 levels by Western-Blotting method at one and two weeks.Result:1. The MTS values of osteoblasts gradually increased, which showed the cells’ proliferation on the surfaces of coatings.There was a significant difference in the proliferation of osteoblasts in different coatings (p<0.01), which showed that the mineralized collagen coating could significantly promote cell proliferation.2. Scanning electron microscope observation showed that the osteoblasts could grow on the surfaces of all the coatings at 24h.In dense mineralized collagen coating group, cells grew better than other groups,spreading and extending pseudopodia, fixing on the surfaces of the materials.3. Western-blotting was used to detect the:ALP and COL-1 expressions of osteoblasts on mineralized collagen coatings were higher than those of pure Ti group after one week’s culture. ALP expressions of (Compact type) mineralized collagen coating group were higher than those of porous coating group. After two weeks, the COL-1 expression levels of mineralized collagen coating group were significantly higher than those of the pure Ti group.Conclusion:The mineralized collagen coating on titanium surface can promote the adhesion, proliferation and differentiation of MC3T3-e1 cells,and the (Compact type) mineralized collagen coating is more ideal.