Impact Of Electricity Against Cerebral Ischemia Reperfusion Injury In Rats Bcl-2 Bax Gen

Objective:By observing electric ischemia-reperfusion Bcl-2, apoptosis related genes in rats, Bax expression in Brain explore acupuncture after cerebral ischemia improve the regulation of apoptosis, with a view to acupuncture treatment of ischemic Interpretation of effector mechanisms of stroke provide a scientific basis.Method:Choose SD rats according to random number table 24 for control group, the remaining animals modified Longa suppository method is adopted to establish the cerebral ischemia reperfusion model. To building a successful randomized in rats, model group, the electric acupuncture group, respectively, effective number of cases in each group of 24 only. Control in addition to don’t wear into the line switch, other treatment is the same with the model group; Preparation model of ischemia model group 2 h after drawing wire plug, without any treatment process; Electric acupuncture group with disposable acupuncture needle selection "Dazhui" and "Baihui", "Baihui" flat, "Dazhui" Angle of 30° thorn, depth of about 1 cm, meet asaps HANS-200 type electric acupuncture apparatus, negative "Baihui", "Dazhui" to the anode, select the density wave, the frequency for 2/15 HZ, the intensity of stimulus to the needle handle slightly vibrate, rats don’t bark for degrees, continuous treatment for 30 min.24 h after cerebral ischemia reperfusion, consult Julio’s 18 4-point scale method for neural functional behavior scale; Using wet heavy weighing method to calculate the brain water content, the brain water content (%) = (wet weight, dry weight)/wet weight by 100%; Using the immunohistochemical method and rt-pcr technique defect was detected in the lateral cortex Bcl-2, Bax protein expression and the Bcl-2 mRNA, Bax mRNA expression changes.Result:(1) Cerebral ischemia reperfusion injury in rats nerve function score significantly decreased, compared with the control group significant difference (P<0.01); Electric acupuncture intervention can effectively improve the score value, compared with model group there were significant differences (P<0.05).(2) Brain ischemia-reperfusion injury rats water content were significantly higher than the control group (P<0.01), compared with model group electric acupuncture intervention afterbrain water content decreased significantly (P<0.01), and were close to the control group (P>0.05).(3) Defect in side the Bcl-2 cerebral cortex, the expression of Bax protein, model group were obviously higher in the control group (P<0.05 and P<0.01), with the rise of Bax protein is greater than the Bcl-2 protein, the ratio of the Bcl-2/Bax protein decreased (P<0.01); The curative group compared with model group, the Bcl-2 protein expression increased (P< 0.05), the expression of Bax protein reduced (P<0.01), the Bcl-2/Bax protein ratio increased (P< 0.01).(4) The defect in the lateral cortex Bcl-2 the expression of mRNA and BaxmRNA, model group were obviously higher in the control group (P<0.05), the Bcl-2 mRNA/Bax mRNA ratio decrease (P<0.05); Compared with the model group, EA group increased Bcl-2 mRNA was not obvious (P> 0.05), but Bax mRNA decreased significantly (P<0.05), the ratio of the Bcl-2 mRNA/BaxmRNA elevated (P<0.01).Conclusion:Electric against ischemia reperfusion injury in the rat brain the Bcl-2, Bax gene regulation function, improve the ratio of the Bcl-2/Bax, make advantage of antiapoptotic gene, reduce the apoptosis of ischemia-reperfusion area, alleviate cerebral edema, improve nerve function.