| Objective:To investigate the oxidative stress regulatory mechanism of Sophora tonkinensis Gagnep. compatibility with Glycyrrhiza uralensis Fisch. and protective effects through relieving the hepatotoxicity induced by Sophora tonkinensis Gagnep. in mice, and then to research the anti-inflammatory and analgesic efficacy of Sophora tonkinensis Gagnep. compatibility with Glycyrrhiza uralensis Fisch..Methods:1. The acute toxicity LD50 was measured in mice administrated Sophora tonkinensis Gagnep. water decoction, to determine the hepatotoxicity induced by Sophora tonkinensis Gagnep..2. SPF KM mice were randomly divided into five groups:control group, the Sophora tonkinensis Gagnep. group, the Sophora tonkinensis Gagnep. compatibility with Glycyrrhiza uralensis Fisch.1:1,1:2 and 2:1 group. The Sophora tonkinensis Gagnep. group was intragastric administrated Sophora tonkinensis Gagnep. water decoction and the Sophora tonkinensis Gagnep. compatibility with Glycyrrhiza uralensis Fisch.1:1,1:2 and 2:1 groups were intragastric administrated 24 g·kg-1、36 g·kg-1、36 g·kg-1 mixture drugs of Sophora tonkinensis Gagnep. Compatibility with Glycyrrhiza uralensis Fisch., daily for 21 days, respectively. After 21 days, all mice were sacrificed to obtain the serium and liver tissue.2.1 The activity levels of ALT, AST and ALP in the serum were detected by fully automatic biochemical detector.2.2 The content of SOD, GSH and MDA in liver homogenate was evaluated by TBA, WST-1 and colorimetric method, respectively.2.3 The difference of mice liver morphology was observed by HE staining method.2.4 The protein expression of Nrf2 in mice liver was determined by immunohisto-chemical method3. SPF KM mice were randomly divided into five groups as "Methods:2" described, and mice were operated with murine pain pattern model by acetic acid, and ear edema model by dimethylbenzene. The pain inhibition rate and swelling degree of ear in mice were calculated.Results:1. The LD50 of SPF KM mice administrated Sophora tonkinensis Gagnep. water decoction was 47.918 g·kg-1 (95% fiducial limit was 45.192～50.665 g·kg-1).2.1 Compared with the control group, the levels of ALT, AST, ALP were increased in the Sophora tonkinensis Gagnep. group (P<0.05 or P<0.01 or P<0.05). The levels of ALT, AST, ALP in combination groups were decreased than Sophora tonkinensis Gagnep. group(P<0.05), of which the effects of 1:2 group were better.2.2 Compared with the control group, the liver index and the content of MDA were increased in the Sophora tonkinensis Gagnep. group, while the activities of SOD and GSH were significantly decreased (P<0.01). The combination group degraded the content of MDA and the liver index (P<0.05), while increased the activities of SOD and GSH than Sophora tonkinensis Gagnep. group (P<0.05 or P<0.01), of which the effects of 1:2 group were better.2.3 The liver tissue had a series of pathological lesions in Sophora tonkinensis Gagnep. group and combination groups mice. But the combination groups reduced the liver lesions.2.4 Compared with the control group, the protein expression of Nrf2 of liver in Sophora tonkinensis Gagnep. group and the combination groups was significantly increased(P<0.05 or P<0.01). And combination groups higher expressed Nrf2 than Sophora tonkinensis Gagnep. group. The effect of the combination group of 1:2 was better(P<0.05).3. Compared with the control group, Sophora tonkinensis Gagnep. group and combination groups extended the perception time of pain, reduced the times of twisted body and relieved the swelling degree of mice ear (P<0.05 or P<0.01 or P<0.01).It was not statistical difference in pain and inflammation inhibition rates between Sophora tonkinensis Gagnep. group and combination groups(P>0.05).Conclusions:Glycyrrhiza uralensis Fisch. possibly through inhibiting oxidative stress to relieve the hepatic injury induced by Sophora tonkinensis Gagnep., while can not relieved the effect of anti-inflammatory and analgesic effects by Sophora tonkinensis Gagnep.. |
PDF Full Text Request