Study On The Qualitative Evaluation Of Livistona Chinensis(Jacq.)R.Br.

The Livistona chinensis（Jacq.）R. Br. which belongs to palmae is one of the traditional Chinese medicine. It is usually used to treat different kinds of cancer in China. Pharmaco research indicated that it has significant activity of anti-cancer and the main active fraction is the ethyl acetate seperation of alcohol extraction. Presently, some of Chinese traditional patent medicines primarily consisted of Livistona chinensis（Jacq.）R. Br. have developed to the clinical treatment of gastric cancer and liver cancer. But only a few study has been done on the methods of quality evaluation of Livistona chinensis（Jacq.）R.Br., such as morphological, microscopical and TLC identifications. Therefore, the purpose of this paper is to improve the quality control methods for Livistona chinensis（Jacq.）R.Br.ObjectiveIn this study, the methods including thin layer chromatography（TLC） identification, ultra performance liquid chromatographic（UPLC） fingerprint chromatogram, ultra performance liquid chromatography-time of flight mass spectrometry（UPLC/Q-TOF-MS）, determination by ultra perfomance liquid chromatography were researched for the quality evaluation of Livistona chinensis and Livistona chinensis extracts.Methods1. The Livistona chinensis TLC identification methods were researched to choose the best TLC identification method. The tested sample solution of preparation methods, seperate system and chromogenic agent were further optimized. And the new TLC identification method was carried out methodology study.2. The UPLC fingerprint chromatogram of Livistona chinensis exctraciton was obtained with Agilent SB-C18 column （100 mm×2.1 mm,1.8μm） and gradient eluted with acetonitrile and 0.1% formic acid; The flow rate was 0.35 ml·min-1, and the column temperature was maintained at 35℃. The detection was set at: 264 nm （0-6 min）,351 nm （6.01-24 min）,264 nm （24.01-30 min）.10 batches of Livistona chinensis extracts were determined by UPLC fingerprint chromatogram and evaluated by similarity analysis.3. The UPLC fingerprint chromatogram of Livistona chinensis was obtained with Agilent SB-C18 column （100 mm ×2.1 mm,1.8μm） and gradient eluted with acetonitrile and 0.1% formic acid; The flow rate was 0.2 ml·min-1, and the column temperature was maintained at 35℃. The detection was set at:264 nm （0-9 min）,351 nm （9.01-25 min）,264 nm （25.01-40 min）.10 batches of Livistona chinensis were determined by UPLC fingerprint chromatogram and evaluated by similarity analysis.4. The chemical components of Livistona chinensis were researched with UPLC/Q-TOF-MS at negative mode.5. The method of UPLC quality determination of Livistona chinensis was obtained with Agilent SB-C,8 column （100 mm ×2.1 mm,1.8μm） and gradient eluted with acetonitrile and 0.1% formic acid; The flow rate was 0.2 ml·min-1, and the column temperature was maintained at 35℃. The detection was set at: 259 nm、279 nm.Results1. The TLC identification method of Livistona chinensis was established, and the result of methodology study showed that this method had good applicability.2. There were 29 common chromatographic peaks which selected from the UPLC fingerprint chromatogram of Livistona chinensis exctracts. A referential fingerprint chromatogram of Livistona chinensis extracts was established. All of the results of 10 batches evaluated by similarity analysis were more than 0.9.3. The UPLC fingerprint chromatogram of Livistona chinensis was marked 20 common chromatographic peaks and a referential fingerprint chromatogram of Livistona chinensis was established. All of the results of 10 batches of Livistona chinensis researched by similarity analysis were more than 0.9, except one batch of sample.4.16 chemical compounds were preliminary identified using the UPLC/Q-TOF-MS from Livistona chinensis exctraciton. There were 2 chemical compounds which were firstly reported that protocatechuic aldehyde and catechin were consisted in Livistona chinensis.5. The protocatechuic acid and protocatechuic aldehyde performed a good linear relationship witin 0.00106～0.106 mg·ml^-1,0.000279-0.0279 mg·ml^-1 and the average contents of protocatechuic acid and protocatechuic aldehyde were 0.022%,0.0051%.ConelusionThe quality evaluation of Livistona chinensis were studied systematically in this paper that it was improved the methods of the quality control of Livistona chinensis.