Objective:To research the degree of immune graft rejection, the quantity and function of regulatory T cells in the model of liver transplantation after mice liver transplantation to provide theory evidence in revealing mechanisms of immune tolerance.Method:Transplantation liver samples were collected at 3d,7d,14d,28d after allogeneic or syngeneic mice liver transplantation (N=4 for each group at one time point). Graft rejection score was given with HE staining. T and B lymphocytes and change of regulatory T cells were identified with IHC staining.The change of Foxp3 mRNA and CLAT-4 on surface of regulatory T cells were detected by real-time PCR and IFC respectively.Results:Allogeneic graft was rejected during 2w after transplantation, and the rejection receded generally after 2w. Inflammatory cells intra-graft was mainly T lymphocytes. Foxp3 positive cells and mRNA of Foxp3 in allogeneic graft group was significantly higher than these in isogeneic graft group (15.0±1.3vs.2.7±1.0,P<0.05) Meanwhile, expression of CLAT-4 on regulatory Tcells also was higher in allogeneic graft group compared with isogeneic graft group(53%±3% vs.13%±2%, P<0.05).Conclusion:The immunity rejection after allogeneic liver transplantation achived by regulatory T cells negative regulation and then the achived immune tolerance. |