| Objectives:To understand the relationship between blood lead levels and plasma ALA levels in children in a lead contaminated area. To compare the effects of different subtypes of ALAD on blood lead and plasma ALA levels, and providing a clue for the mechanism of the effect of ALAD gene polymorphism on the toxicity of lead toxicity.Methods:This study used cross sectional study,282 (160 males and female 122) children aged 7-9 in a lead pollution area in Yunnan Province were recruited. According to 2006, China’s Ministry of health the children lead high viremia and lead poisoning classification and principles of management of (Trial) ", the research object is divided into control group (BPb< 10 μg/dL), exposure group (BPb ≥10 μg/dL). According to the results of ALAD gene polymorphism analysis, the lead exposed children were divided into two groups:the ALAD11 genotype group and the ALAD 12/22 group. BPb, ALA-P and ALAD gene polymorphisms were detected in venous blood collected from all the subjects.Results:1 The concentration of BPb in lead exposed children was 7.0±10.8 μg/dl (2.5-53.1 μg/dl), the concentration of ALA-P was 5.9±3.1 μg/L (1.2-46.6 μg/L).2 Exposure group ALA-P level (6.27 ± 2.07 μg/L) was higher than that of the control group (5.54±1.65 μg/L), the difference was statistically significant P<0.05.3 ALAD11 genotype was 92.91%, ALAD12/22 was 7.09%, no ALAD22 genotype was found, the frequency of allele ALADi and ALAD2 were 96.45%and 3.55%, respectively.comply with the law of Hardy-Weinberg balance.4 In the detection of 282 lead exposed children with ALAD genotype,160 males and 122 females, mean age (8.06±1.117), age range (9-12). The x2/ttest of different subtypes of lead exposed children’s gender and age were not statistically significant.5 According to Spearman correlation analysis, exposure to lead children BPb and ala-p level was positively correlated rs=0.181, P< 0.05; ALAD11 gene type of lead exposure to children BPb and ala-p level was positively correlated rs=0.201, P< 0.05; ALAD 12 genotype lead exposed children BPb and ala-p level no correlation (P> 0.05).6 ALAD11 gene lead exposure in children with BPb levels (7.09±10.32 μg/dL) were higher than that of children with ALAD12 genotype (5.79 ± 5.31 μg/dL), with no statistically significant difference between P>0.05.ln the control group, the levels of ALAD11 in children with BPb exposure (5.61±2.45 μg/dL) were higher than those of the ALAD12 genotype children (5.43±1.37 μg/dL), and there was no significant difference between and P>0.05. The exposure group ALAD12 gene lead exposed children’s BPb level (20.07 ±12.33 μg/dL) is higher than the ALAD11 genotype children (17.05±17.35 μg/dL), no statistically significant difference P>0.05.7 ALAD11 gene type of lead exposure ala-p (5.97±3.14 μg/L) level in children is higher than that of children with ALAD12 genotype (5.43 ±μ1.61 μg/L), the difference was not statistically significant difference (P> 0.05); control group ALAD11 gene type of lead exposure ala-p (5.33 ± 1.72 μg/L) level in children is higher than that of ALAD12 genotype children (5.26 ± 2.63 μg/L), the difference was not statistically significant difference (P> 0.05); exposure group ALAD11 genotype lead exposure ala-p (6.22±2.15 μg/L) level in children is higher than that of ALAD12 genotype (4.73±1.02 μg/L), the difference is statistically significant difference (P< 0.05).8 Multiple linear regression analysis showed that gender, age and ALAD genotype were not the influencing factors of BPb, and ALA-P was the influencing factor of ALA-P (β=0.052,P=0.007) Conclusions:1 BPb levels were positively correlated with ALA-P levels in children with lead exposure, P<0.05. By multiple linear regression analysis, BPb is the main influencing factor of ALA-P. Suggest that the level of ALA-P in children with lead exposure is mainly affected by the level of blood lead.2 No association was found between ALAD polymorphism and BPb levels in children with environmental lead exposure.3 In children with high levels of lead exposure, the ALAD11 genotype was higher than ALAD 12 genotype in individuals with ALA-P genotype. Suggested that ALAD gene polymorphism as a susceptibility biomarker may be used as a screening index for high ALA levels in children with high exposure to environmental exposure. |
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