| PrefaceHuman beings have used lead for thousand years, along with it is lead intoxication. Because of its wild use, research of lead intoxication stills plays an important role in heavy metal toxicology.Producing conditions of lead are advancing as the development of science. Lacking of high - concentration lead environment makes the epidemic researches of developed countries pay more attention on healthy effect induced by lead of low -concentration. But in China, especially for small enterprises, the working condition is terrible, so the healthy risk caused by exposed to high - concentration lead can't be ignored at all. Investigation of Shenyang Smelting Factory which technology is only equal to the early 70'slevel in developed countries, in-dicat that the lead concentration in working environment is very high. Workers in Shenyang Low - Pressure Switch Factory were studied as a group exposed to low - concentration lead, who were exposed the smoke from Smelting Factory. Though they didnt take part in the smelting work, they were worked in high -concentration air. This study is based on this background.Dose - effect relation can be seen in the early stage of lead intoxication, with the more exact and sensible analyzing methods. The revaluing the dose -effect relation between lead and porphyrin metablism is very important to defining the up - limit and diagnosis of " lead worker" and it is good for healthy care, healthy diagnosis and drafting healthy standards.We dont know exactly how much lead can effect blood pressure. China is under high - rate hypertension, and it is very important to prevent hypertensionfrom prevalence. Whether lead can cause hypertension still needs more evidences.Research of ALAD genetic polymorphism is the key - point of lead influence to porphyrin metablism, Also, it is another aspect of the sensitivty of human gene to occupational and environmental factor. This is important to definition of employees and high - risk group.Material and MethodsIn this research, we chose 150 workers from Smelting Factory and 70 workers from Switch Factory as object, all were males and average ages were 36. 00 ±1.31 and 33.54±8.49. The questionnaire were based on " Questionnaire of Worker Exposed to lead in Shenyang" , added some symptoms, medical record, family history, smoking history, drinking history, recuperation and chelating lead and health custom of worker in working places. We detected lead in blood, ALAD,ALA - P,ALA -U, Ht and Hb in 5ml peripheral blood. We also detected the blood pressure of the objects. As susceptive group we chose 11 low -blood - lead and larger decreasing ALAD values objects in 230 samples to analyze ALAD gene polymorphism.We used no - flame atom absorption spectrophotometer, colorimeter and HPLC to detect blood lead, ALAD, ALA - P and ALA - U, and used CY. XJl 1. 300 blood - Pressure meter to detect blood pressure, separated the WBC of sensible group, extracted DNA and then made PCR amplify by specific primer. We compared ALAD genotype to the standard marker after making gel - e-lectrophoresis, analyzed these results by SPSS10.0, then did ANOVA analyzed, t - test, correlation, regression, x2 ~test and multiple - regression.ResultsThe research indicated that ALAD had statistic decreasing when the lead concentration of blood was 15 -20μg/dl and ALA - P, ALA - U was at 20μg/dl, the concentration increased. Correlation coefficient of blood lead and LOG10 ALAD, LOG10ALA - P, and LOG10ALA - U are -0. 893,0. 853,0. 867. Its liner regression were y = 0. 4055 - 0. 018x, y = 0. 2148 + 0. 0221x, y = - 0. 3417 +0. 019Ix. But there was no obvious relationship between blood lead and Ht, Hb. Good correlation coefficient existed in blood lead and air lead, r=0. 802. Correlation coefficient of air lead and LOG10ALAD, LOGGIA - P, and LOG10ALA - U and Ht were - 0.778,0.725 ,0. 760 and - 0.152,liner regression regulation were y =00. 1155 -0. 6187x, y =0. 6427 +0.7343x, y = -0. 0397 +0.6570x and y =47. 5429 -0. 8279x. Obvious increasing SBP,...
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