Fluorescence In Situ Hybridization For Detection Of Epidermal Growth Factor Receptor Gene Amplification On Slides From Rapid On Site Evaluation In Non-small Cell Lung Cancer

Objective This study collected the dissecting material fixed on slides from rapid on site evaluation(ROSE) of non-small cell lung cancer(NSCLC) patients who were treated at Tianjin medical university general hospital, and at the same time, we got the relevant information about clinical manifestations of these patients. Aim to investigate the detection of epidermal growth factor receptor(EGFR) gene amplification status on the smears from ROSE use the method of fluorescence in situ hybridization(FISH) in NSCLC, and to research the the relationship between the EGFR gene amplification status and the clinical characteristics(age, gender, smoking status, histological type, degree of differentiation, tumor stage correlation) in NSCLC.Methods From the September 2013 to December 2014, we totally collected the 71 cases of dissecting material fixed on smears from ROSE, whose histological type has been proofed by the Department of pathology, and use of FISH technology to detect EGFR gene amplification status. The results were compared with clinical characteristics of patients. Application SPSS16.0 statistical software processing, P < 0.05 for the difference was considered statistically significant.Results 1. In our study, the number of ROSE(+) and diagnosed of NSCLC was 72 cases. Only 3 of them failed confirmed the pathological type because of fewer specimens obtained. Another 2 cases were considered as small cell lung cancer on the smears from ROSE, but pathology finally diagnosed as poorly differentiated squamous cell carcinoma,in our study,the the diagnosing rate of ROSE was 95.9% in NSCLC. And furthermore, false negative results does not appeared. At the last, we collected 71 specimens from NSCLC patients. 2. In the 71 patients, a number of 68 cases successfully achieved results, in which the number of EGFR gene amplification, namely FISH(+) was 23 patients, with 7 were clustered amplification. The overall amplification rate was 33.8%. The other 3 cases failed because of less of cell or a number of overlapped was too much or the low hybrid rate. And EGFR gene amplification status wasn’t associated with the age(χ2=0.176,P=0.675), gender(χ2=2.796,P=0.094), smoking status(χ2=0.584,P=0.445), pathological type(χ2=1.248,P=0.264), degree of differentiation(χ2=1.234,P=0.267), tumor stage(P=0.474)(all P> 0.05).Conclusions This study used FISH technology to detect the EGFR gene amplification status on the smears from ROSE, the results showed that:1.FISH could be applied on the smears from ROSE to detect EGFR gene amplification status. Compared with traditional methods, it was more simple and economical. On the other hand, we should improve the quality of slice production to make sure the FISH procedure could be carried out smoothly. 2.The EGFR gene amplification were identified in 23 of 68 samples(33.8%). And it was not associated with the age, gender, smoking status, pathological type, degree of differentiation and tumor stage, namely it was not associated with the clinical characteristics.