Effects Of Probiotics On Intestinal Immune And ICC Of Colon Cancer Patients

Objectives: To investigate the effect of probiotics on the intestinal immune and ICC of colon cancer patients, to provide theoretical evidence for clinical application of probiotics.Methods: ①The colon cancer scheduled to undergo right hemicolectomy of hospitalized patients as research subjects, randomly divided into control group and the probiotic group, probiotic group giving probiotics drug oral 3 days before surgery, and control group was not special treatment. ②Comparison of the two groups research objects postoperative infection, postoperative 24 h WBC. ③ HE staining to observe research object ileum and colon inflammatory cell infiltration and goblet cell changes. ④Immunohistochemical detection expression of IL-10, IL-17, IL-22 of research objects’ ileum and colon tissue, and calculate mean density. ⑤Enzyme-linked colorimetric test the concentration of D-lactic acid of research objects’ blood. ⑥Comparison of the two groups postoperative gastrointestinal recovery, and immunohistochemistry to detect the expression of c-kit of research objects’ ileum and colon tissues.Results: ①3 cases of control group and 2 cases of probiotic group had postoperative infection. 24 h after operation the control group and the probiotic group WBC were(10.26±2.81)×109/L and(9.40±2.12)×109/L. Percentage of neutrophils were(81.87±8.45)% and(77.16±7.40)%. No significant difference between them(P>0.05). ②Compared with the control group, probiotic group infiltration of inflammatory cells in Ileum and colon tissue was mild. The number of goblet cells was(5.93±1.44)×10-5 and(6.70±1.07)×10-5 more than control group(4.85±1.37)×10-5(P<0.05)and(5.76±1.26)×10-5(P<0.05)respectively. ③ IL-10 mean density of probiotic group in ileum and colon tissue were 0.1550±0.0308 and 0.1713±0.0396, significantly higher than control group 0.1307±0.0337(P<0.05) and 0.1442±0.0303(P<0.05). IL-17 mean density of probiotic group in ileum and colon tissue were 0.1461±0.0339 and 0.1323±0.0283, significantly lower than control group 0.1751±0.0378(P<0.05) and 0.1552±0.0302(P<0.05). IL-22 mean density of probiotic group in ileum and colon tissue were 0.1184±0.0201 and 0.1212±0.0225,significantly lower than control group 0.1374±0.0272(P<0.05)和 0.1480±0.0385(P<0.05). ④D-lactate concentration of probiotic group is(603.6188±56.8916)μg/L, significantly lower than control group(885.7639±106.0899)μg/L(P<0.05). ⑤There is no difference between control group(4.45±1.05)day and probiotic group(4.55±1.39)day in defecation after surgery(P>0.05). Expression of c-kit in ileum and colon tissue of probiotic group was significantly higher than that in control group.Conclusions: ①Probiotics reduce the extent of inflammatory cell infiltration in the ileum and colon intestinal mucosal tissue of colon cancer patients, protect the number and normal function of goblet cells. ②Probiotics can increase the expression of inhibition inflammatory cytokine IL-10 in ileum and colon tissue of colon cancer patients, reduced the expression of inflammatory cytokines IL-17, IL-22. Thereby reducing the local intestinal inflammatory response. ③ Probiotics can reduce blood D-lactate levels in patients with colon cancer. Probiotics can reduce the permeability of the intestinal mucosa of colon cancer patients, enhanced mucosal barrier. ④Probiotics can increase the expression of c-kit in ileum and colon tissues, increase the number of ICC, it may be a factor in probiotics can regulate gastrointestinal motility.