| Continuous development of modern medicine, resulting in the extraction process of the active ingredient in traditional Chinese medicine as well as to determine the content has become a new hot spot. Synthesized and purified Paeonol do quantitative analysis, due to the complexity of the test system components, usually after separation or reaction quantitatively, to bring some difficulties to detect. Through the establishment of quantitative analysis method is not an isolated multi-component mixtures. Using UV spectroscopy combined with vector-subspace projection oblique angle criterion algorithm and theory, to achieve an accurate analysis of the sample Paeonol content. Enabling rapid analysis of samples, and applies these two methods Paeonol thermodynamic study proposes a new Paeonol solvent Crystallization processes to increase production, and thus the rapid analysis method is applied to the crystallization process monitoring. Research Topics include:(1) The HPLC-UV method combined with the space angle criterion established paeonol rapid analysis methods. By HPLC-UV combined with acquisition Paeonol crystal liquor time, wavelength, absorbance of three-dimensional data, construction without measured component.paeonolum deduction criterion by vector background database. The results show that: The results of this method and HPLC analysis, the relative error is less than 2.67%, and the recovery was 97.33% ~ 102.5%. After the analysis model, only a single acquisition of sample solution to complete the ultraviolet spectrum analysis. The method is rapid, accurate, and can be Paeonol crystalline product quality and control of various formulations to provide practical solutions and to prove that the method can be used for rapid detection Paeonol crystallization process.(2) Using the HPLC-UV method with oblique projection algorithm combines established Paeonol content rapid analysis methods. Acquisition Paeonol crystallization mother multi-wavelength UV spectrum 3D data measured components were constructed without phenol and Dan background database contains only test database Paeonol component, calculated using oblique projection method, the sample paeonol were determined. After building the analysis model, simply collect the UV spectrum of the sample solution can be achieved paeonolum quantitative analysis. The results are approximate and HPLC results. Meanwhile oblique projection method can also be used for rapid determination of paeonol purity, which is an extension of the oblique projection methods, without the need for sample dilution accurately weighed and fixed proportion, just a sample diluted to a certain concentration range, to meet effective collection ultraviolet spectrum, you can paeonol purity samples were analyzed by HPLC and the results approximate the results prove that the method can be used to quickly detect paeonol product purity.(3) Rapid analysis method applied to the determination crystalline established thermodynamic data of paeonol, Investigation Paeonol metastable zone width in 75% ethanol solution and Paeonol solubility in aqueous solutions of different concentrations of ethanol. The data is discussed, and the feasibility of using the Dilution Crystallization Purification paeonolum.(4) Dilution Crystallization method using, for Paeonol crystalline factors inspect, given a final yield of indicators optimum conditions. Dilution Crystallization of products to explore washing conditions, in order to obtain pure Paeonol products, establish a complete Paeonol crystallization process. And oblique projection method was applied to the rapid detection of product quality, the vector- subspace angle criterion analysis method is applied to crystalline process monitoring. |
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