Reaserch On Key Technology Of Anaylzer For Red Blood And Platelets Based On Microfludic Chips

Blood analysis is one of the commonly used clinical detection methods, which test result is the main criteria of diseases such as infection, inflammation and allergy and etc. In recent years, with the continuous development of microfluidic chip technology, as well as the growing demands of small volume, light weight, low power consumption, simple operation portable blood analyzer system which capable of the real-time detection, micro blood analysis system based on microfluidic chip received more and more attention. This research combined microfluidic chip with laser scattering method. According to light intensity difference between the red blood cells and platelets by laser light scattering, a low cost, miniaturization of blood cell analysis system was established. It is capable of counting red blood cells and platelets. The research work could provide some experiences for the development of miniaturizing, low cost, portable red blood cells and platelets analyzer. The main works of the research includes the following aspects:1) Designing and fabricating microfluidic chips. It can decrease the sample and reagent consumption, and reduce the volume and cost of the device.2) Building an orthogonal optical path device. Using a small and low cost semiconductor laser as a light source, one 10 X microscope objective was used to focused laser beam, combined with a lenticular lens and diaphragm to implement the collection of scattered light. A photodiode was used to detecting scattered light signal.3) Processing signals. Using a data acquisition module for the scattered light signal acquisition and transmission, an upper computer program was programed to complete signal processing, display, analyze and storage functions.4) Testing the system. Using 4μm and 10μm polystyrene microspheres as detection objects, the performance of the system was evaluated. The system detected about 43 samples per second. The coefficients of variation for 4μm and 10μm polystyrene microspheres were 33% and 30% respectively. The two microspheres classification and counting can be achieved.