The Related Research About Monocyte Chemotactic Protein-1 Gene Polymorphism And Spontaneous Bacterial Peritonitis In Hepatic Cirrhosis Patients

Objective:To investigate the association of the functional monocyte chemotactic protein-1 promoter polymorphism(A-2518G) and spontaneous bacterial peritonitis in patients with hepatic cirrhosis ascites.Methods: From November 13, 2014 to November 31, 2015, 80 cases of inpatients with hepatic cirrhosis ascites that they were not for anti-infection treatment were collected from the Sixth People’s Hospital of Dalian. They were divided into 2 groups, namely 40 cases of patients with SBP, 40 cases free from SBP. In addition, there were 30 cases of healthy blood donors as a healthy control group. Venous blood was drawed from the patients in both groups and healthy control group, and stored in the-70℃ refrigerator. By whole blood genomic DNA extraction, target gene PCR amplification reaction, restriction enzyme digestion reaction, agarose gel electrophoresis to obtain the MCP-1 gene types, and using statistical analysis to study the correlation between MCP-1 gene polymorphism and SBP in patients with hepatic cirrhosis. At the same time, the clinical data were collected from 80 cases of hospitalized patients, and using statistical analysis to observe the relationship between them and SBP in patients with hepatic cirrhosis.Results: The frequency of AG genotype in hepatic cirrhosis with SBP group was significantly higher than that in healthy control group, healthy control group 12(40%) vs SBP group 30(75%), P<0.05, while the frequency of GG genotype in hepatic cirrhosis without SBP group was significantly higher than that in healthy control group, healthy control group 3(10%) vs without SBP group 26(65%), P<0.05. When comparing the two groups of patients with each other, a significantly higher frequency of the AG genotype was observed in cirrhotic patients with SBP, SBP group 30(75%) vs without SBP group 8(20%), P<0.05, while a significantly higher frequency of the GG genotype was observed with cirrhotic patients without SBP, SBP group 6(15%) and without SBP group 26(65%), P<0.05. There was a significantly higher frequency of the G allele in both groups of patients(SBP and without SBP groups) when compared to healthy volunteers,SBP group 42(52.5%) vs health control group 18(30%), P<0.05, without SBP group 58(72.5%) vs health control group 18(30%), P<0.05. When comparing both groups of patients with each other, the A allele frequency of SBP group is higher than without SBP group, namely SBP group and 38(47.5%) and cirrhosis without SBP group 22(27.5%), P<0.05, while The G allele frequency of cirrhosis without SBP group was higher than the SBP group, namely cirrhosis without SBP group 58(72.5%) and SBP group 42(52.5%), P<0.05, and these differences were statistically significant. The levels of albumin, total bilirubin, PCT, serum sodium, and Liver function Child-Pugh grades respectively were statistically significant in patients hepatic cirrhosis with SBP and without SBP.Conclusion: 1. MCP-1 A allele and AG genotype may increase susceptibility to SBP. 2. For patients with cirrhosis of the liver cirrhosis ascites patients carrying the MCP-1 A allele and AG genotype may require regular follow-up and monitoring, and early proceed clinical intervention. 3. levels of serum albumin, serum total bilirubin, PCT, serum sodium, and Liver function Child-Pugh grades respectively were risk factors of SBP.