Expression Of HOXB4、PRDM16 And HOXA9 In Patients With Acute Myeloid Leukemia And Its Clinical Significance

Objective : To investigate HOXB4,PRDM16 and HOXA9 gene expression in patients with acute myeloid leukemia(AML)and its clinical significance.Methods: Real-time quantitative PCR(RT-q PCR) with SYBR Green assay was used to detect the expression of HOXB4 、 PRDM16 、 HOXA9 gene in AML patients(40 cases),patients with complete remission(9 cases) and patients with non-malignant hematologic diseases(10 cases).The relationship between HOXB4,PRDM16,HOXA9 gene expression levels and clinical features was investigated by statistical analysis.Results: The expression of HOXB4,PRDM16,HOXA9 gene were significantly higher in AML patients than that in the controls(P<0.05). It were observed that the expression of HOXB4 correlate with leukemic blasts in bone marrow, and these three genes correlate with each other. There was statistically significant differences among gene expressions in different course phase(newly diagnosed, relapse, remission).No correlation was observed between expression levels of HOXB4、PRDM16、HOXA9 and chromosome risk status. It was noticed that expression levels of HOXB4,PRDM16,HOXA9 genes were lower in patients who achieved remission after two courses of chemotherapy than the other. And high expression group of each gene had a lower remission rate than that in the low expression group.Conclusion: There are somewhat correlation between the expression level of HOXB4,PRDM16,HOXA9 genes and leukemic blasts and curative effect. The expression of HOXB4,PRDM16,HOXA9 genes is higher in acute leukemia patients,and lower in patients acquired CR/PR.High expression of HOXB4,PRDM16,HOXA9 genes might predict an adverse prognosis.