| To investigate the relevance analysis on the single nucl-eotide polymorphism(SNPs) of Annexin A5(ANXA5),kinase insert domain containing receptor(KDR),MTHFR,MTRR genetic risk of recurrent spontaneous abortion in china han womenMethods: firstly,we screened genes by protein interaction,then det-ermined by Novosnp3.0.1.finaly, genotypes distribution of ANXA5-302T>G(rs1050606),KDR-604T>C(rs55713360) and MTHFRC677T(rs1801133),A1298C(rs1801131)A66G(rs1801394) polymorphism in 190 patie-nts suffered from RSA and 272 normal controls were detected by sequ-encing method.Results: 1. The genotype frequencies of the 4 genes reached the Hardy-Weinberg equilibrium, this indicated that the populat-ion study were selected randomly.2. In controls(274), the frequency of TT、 TG and GG of ANXA5-302T>G were 62.5%、31.6%、5.9%. the frequency of TT、TC and C C of KDR-604T>C were 37.5%、46.3%、16.2%, the frequency of CC、TC and TT of MTHFR C677 T Were 44.1%、53%、5.9%;in RSA group(190),3 genetype of ANXA5-302T>G were found,and TT、TG、GG occupied 54.6%、32%、13.4%, the frequency of TT、TC and CC of KDR-604T>C were26.8%、32%,41.2%, the frequency of CC、TC and TT of MTHFR C677 T Were 64.2%、31.5%、3.2%.Those frequencies of gentype were showed statistically significant between the patient-s andcontrols(P<0.05). The prevalence of the MTHFRA1298 C associate d 3 genotypes and A/C alleles, MTRRA66 Gassociated 3 genotypes we re showed statistically significant between the patients andcontrols(P<0.05).3.The distribution difference of GG,TG+GG genotypes of ANXA5-302T>G between RSA and control groups showed statistically significance(p<0.05), and the relative risks of suffering from RSA in patients c-arrying GG,GG+TG genotypes increased(P=0.027,OR=2.889;P=0.020,OR=1.911respectively); The distribution difference of CCgenotype of K DR-607T>C between RSA and control groups had statistically significa-nce(P<0.05), and the relative risks of suffering from RSA in patients carrying CC genotypes increased(P=0.000,OR=5.231). the frequencies of gentype were MTHFRA1298 C,MTRRA66G were statistically signif icant between the patients andcontrols(P<0.05).4. interactive analysis of gene: There are 3 risk of factors, one of risk factor increase the risk of recurrent miscarriage(OR = 5.095, P =0.000, 95% CI = 2.925- 9.324) of two mutations, increase the risk of r ecurrent miscarriage(OR = 32.694, P = 0.001, 95% CI = 3.592- 229.449), the evidence that the building in a network and the genetic risk of blood clots in the RSA’s hypothesis..Conclusions: The frequencies of genotype and allele of M THFRA1298 C and MTRRA66 G were not statistically significant bet-ween the patients and controls. The polymorphisms of the MTHF RA1298 C and MTRRA66 G local are not associated with recurrent spontaneous abortion. The samples which carried with ANXA5302 GG,T G+GG genotype had an increased risk of RSA than those carri-ed with TT genotype. The samples which carried with KDR607 TT+TC genotype has decrance risk of RSA than thosecarried with CC genotype, the disease risk increased by carrying susceptible CC gentype of the KDR-607, the disease risk increased by carrying susceptible TT gentype of the MTHFR 677,and polymorphicsites of ANXA5-302T>G,KDR-607T>C,MTHFRC677 T are novel risk factors of suffering from R-SA in China Han women. When there is a higher risk for the, increase the risk of recurrent miscarriage(OR = 5.220, P = 5.220, 95% CI = 2.925 9.324) of two mutations, increase the risk of recurrent miscarriage(OR = 28.707, P = 28.707, 95% CI = 3.592 229.449), the evidence that the building in a network and the genetic risk of blood clots in the RS A’s hypothesis.. |
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