The Discussion Of The Hepatitis B Patients Is Prone To The Risk About The Diabetes

Objective:The deficiency linked to immune system response exists in the early stage of DM, and many studies have proved the immune deficiency in the HBV infection patients. At present, there are no definite opinion about the question that chronic viral hepatitis is prone to DM or DM is susceptible to the chronic viral hepatitis infection, therefore the problem and correlation between the immune deficiency of the diseases mentioned above are worthy of progressive research and study. Though the knowledge and understanding connected with DM and chronic viral hepatitis have researched to the level of cell and molecule, and many unknown mechanisms of the pathogenesis need to be explored. Many foreign studies focused on the correlation between chronic viral hepatitis infection and its complications, including DM or insulin resistance, especially concerning with the progressive degree of the liver cirrhosis and metabolism syndrome. We know little about the question of susceptibility to DM during the chronic infection of viral hepatitis B or C, and there are few related studies and reports. We collected the data of the chronic viral hepatitis B patients in detail and have an exploratory research and analysis on the condition of its susceptibility to DM.Materials and Methods:Used by enzyme-linked immunosorbent assay(ELISA), manual or automatic microplate test patients for hepatitis B; for patients with hepatitis B diagnosed by glucose oxidase method to detect hepatitis B patients ’ s fasting blood glucose and two-hour postprandial blood glucose;We used by double urine colorimetry to detect serum total protein, by bromocresol green method to detect serum albumin, by high performance liquid chromatography(HPLC) methodd to detect the glycated hemoglobin(Hb Alc) of patients. Using CYTO FLEX Flow cytometry instrument to detect lymphocyte CD4 and CD8 cells subgroup.2035 cases come from the Tianjin Hai Bin people’s hospital、Tanggu hospital and Hai He hospital,from septermber 2013-2014.These patients examed the hepatitis B by using an enzyme-linked immunoassay microplate(manual or automatic microplate)(diagnosed by People’s Republic of China Department of health. 299-2008 hepatitis B diagnostic criteria for diagnosis). Color not clear of parts to send Tianjin KINGMED CENTER FOR CLINIC LABORATOY using the chemical luminescence detection to determine. At the same time We measured 2035 patients ’ fasting blood sugar and two-hour postprandial blood glucose、a serum albumin、total protein and glycosylated hemoglobin(Hb Alc) detection.Using CYTO FLEX Flow cytometry instrument to detect lymphocyte CD4 and CD8 cells subgroup.In Hai Bin people’s hospital Physical Examination Center we randomly selected 800 case these were tested negative for the hepatitis Markers, no diseases, liver and kidney function is normal, as a control group to detect fasting blood sugar and two-hour postprandial blood glucose、a serum albumin、total protein and glycosylated hemoglobin(Hb Alc) detection.Using CYTO FLEX Flow cytometry instrument to detect lymphocyte CD4 and CD8 cells subgroup.Results:In HBSAg+HBEAg+HBCAb+ group,PFG and postprandial blood glucose and glycated hemoglobin were 5.41 ± 1.32 mmol/L,8.14 ± 2.50 mmol/L,5.49 ± 1.18mg/d L.;CD4(%)33.28±16.23,CD8(%) 30.03±14.56,CD4/CD8 1.68±0.72;total protein/albumin 56.44±5.34/33.96±4.78.In HBSAg+HBEAb+HBCAb+ group,PFG and postprandial blood glucose and glycated hemoglobin were 5.64± 1.66 mmol/L,8.43 ± 3.14 mmol/L,5.65 ± 1.38mg/d L; CD4(%)30.91±12.45,CD8(%)26.28±10.17, CD4/CD8 1.36±0.59;55.17±6.83/31.65±4.14.In Control group PFG, postprandial blood glucose and glycated hemoglobin were 5.08± 0.43 mmol/L,7.42 ± 0.63mmol/L,5.17 ± 0.47mg/d L; CD4(%)40.35±14.88,CD8(%) 38.07±13.62,CD4/CD8 1.26±0.62;62.36±6.15/37.35±6.51Conclusion:Large/small group compared with the control group, FPG/2h Glu 、glycated hemoglobin were extremely significant difference(p<0.01). These dates told us patients with chronic hepatitis B virus infection have a greater impact,It can significantly alter their glucose tolerance status,even may be causing diabetes mellitus. These results are consistent with foreign research. By comparison, we also found that patients with large/small there are also significant differences among the various indicators(p<0.01), shows that different infection status for Glucose tolerance of different effects. Combined with large/small values above the situation, showed HBSAg+HBEAb+HBCAb+ group ’ glucose abnormalities in patients with HBSAg+HBEAg+HBCAb+ group is serious.In this test,the large group、the small group and control group was prone to the risk about the diabetes was 6.7%、5.3%and2%,these consion was consistent with forein report。the incidence of hepatitis b patients is about three times as many normal people, We measured the different groups of total protein, albumin and CD4, CD8 and CD4 / CD8 ratio. Visible differences and HBV infection group, normal control group(big and small) between different infection state its value is different, but not seen after statistical analysis were significant difference(p > 0.05).The results do not exclude data bias may be caused by small sample size of this study. For this difference of internal mechanism, there is need for further discussion and research.