Antibacterial Capability Of Silver Nanoparticles Loaded Titanium Nanotubes Implant Surface Coating

Background:With the development of society, economy and science, implant denture relying on its own advantage has gradually become the preferred method of treatment of dentition defect. At present, titanium and titanium alloy because of its good biocompatibility can form osseointegration be widely used in the field of oral implant. How to improve the success rate of implanting becomes a research hotspot in recent years. Except by changing the implant surface roughness or load growth factor and other methods to enhance implant osseointegration ability, how to suppress the peri implantitis have been paid more and more attention. The oral cavity as a bacterial environment, during the operating process will have bacteria into implant area and have the competition with surrounding cells for implant surface. Once the bacteria on the surface of implant formation biofilm will be difficult to remove, and resist the host immune response, have influence of initial stability with bone and implant. Uniform tubular structure of the nano level can not only increase the implant surface roughness, but also can be used as a drug carrier loading of broad-spectrum antibiotics for preparation of antibacterial coating for local delivery to reduce peri implantitis. Antibiotic is easy to cause the body flora imbalance and emergence of drug-resistant strains has limited its use. Silver nanoparticles as a broad spectrum of inorganic antibacterial agents, not only has the advantages of nano material itself, but also more bacterial inhibition performance, smaller cell toxicity, high safety performance, small volume and easy loading of nanotubes in local slow release implant.Objectives:Successfully prepared by nanometer silver loaded titanium dioxide(Ti O2) nanotubes implant surface coating, detection of different diameter of the titanium dioxide nanotubes drug-loading capacity and different concentrations of silver nanoparticles loaded in nanometer titanium dioxide nanotubes for staphylococcus aureus antibacterial ability difference. To seek the most appropriate of titanium dioxide nanotubes diameter, and the best load drug concentration, effectively restrain around to reduce the adhesion of bacterial resistant bacteria produce around the implant. To titanium dioxide nanotubes as drug carrier local drug delivery in the surface of implant, implant surface coating inhibit bacterial adhesion and proliferation to provide theoretical basisMethods:Using anodic oxidation method respectively under 10 V and 18 V in order to make an orderly of titanium dioxide nanotubes with different diameter. Testing specimen surface morphology by scanning electron microscope. Preparation of nanometer silver colloid by reduction method, through transmission electron microscope test particle size. Through using the method of in situ replacement take silver nanoparticles into titanium dioxide nanotubes. The surface morphology of silver nanoparticles loaded titanium dioxide nanotubes detecting by the scanning electron microscope, X-ray photoelectron spectrometry and transmission electron microscope. Calculation the minimum inhibitory concentration of nano silver solution. Testing all the samples hydrophilicity before and after ultraviolet irradiation. Using bacteriostatic plate count method test the number of planktonic bacteria andadhersion bacteria after Staphylococcus aureus cultured on silver nanoparticles loaded titanium nanotubes surface one day, three days, five days. Inhibit bacterial adhesion ability detecting by scanning electron microscopy and laser scanning confocal microscope. Results:This experiment successfully prepared titanium dioxide nanotubes which loaded diameter of 20 nm silver nanoparticles in. After UV irradiation formed more hydrophilic silver nanoparticles loaded titanium nanotubes surface, and can inhibition Staphylococcus aureus adhesion and proliferation in three days. Nanometer silver loaded titanium dioxide nanotubes antibacterial capacity increases with the concentration of nano silver solution. 18 V voltage prepared 90-110 nm diameter titanium dioxide nanotubeshave better carrying silver nanoparticle ability and better inhibition of bacterial adhesion ability than 20-30 nm diameter titanium dioxide nanotubes nanometer prepared by a voltage of 10 V.Conclusions:Voltage of 18 V prepared titanium dioxide nanotubes loaded 100 m M nano silver solution has the best inhibiting Staphylococcus aureus adhesion and proliferation properties.