| Objective For a long time, how to develop biomaterials with good blood compatibility has been a difficult and hot problem in blood perfusion. In recent years, plasma exchange combined with plasma perfusion for treatment in patients with severe jaundice, though the formed elements in the blood can be avoided direct contacting with the adsorption material, improving its blood compatibility directly, it has greatly increased the cost of patients, the technology is also relatively complex. These limitations made some difficult in clinical. In order to save costs, reduce the economic burden of patient, we made a study on the blood compatibility of HB-H-6 resin by prefilled albumin and PDMS membrane. The blood compatibility, cost-effectiveness and the operation of three kinds of HB-H-6 resin, including untreated HB-H-6 resin, prefilled albumin resin and the resin coated by PDMS membrane, were compared with each other, hoping to find a simple and cheap way to improve the blood compatibility of HB-H-6 resin, and to provide a theoretical basis for whole blood perfusion in clinical directly.Methods This experiment is divided into three parts. In the first part, we simulated the adsorption process by the dynamic and static adsorption in vitro experiment, analyzed the changes of common index between the whole blood and plasma before and after adsorption, and examined the adsorption rate of electrolytes, protein, complement and other indicators in whole blood and plasma in HB-H-6 resin untreated group, to judge whether the whole blood perfusion can replace plasma perfusion in clinical or not. The second part, adsorption was carried out on the whole blood by albumin prefilled resin and untreated resin, we analyzed the changes of each index before and after the adsorption. Then we made a contrast on the effects of the albumin prefilled resin and untreated resin on blood cells and platelets in whole blood. The third part, we coated the HB-H-6 resin by PDMS membrane, and made a static adsorption experiment on whole blood in vitro. Then we made a contrast on the blood compatibility of the HB-H-6 resin untreated, HB-H-6 resin prefilled albumin and HB-H-6 resin coated by PDMS membrane.Result The first part of the experimental results show that: dynamic and static adsorption experiment results show that the protein in blood plasma and whole blood group has no obvious change(p>0.05) before and after the adsorption, C3 and C4 has a certain change, but not obviously(p>0.05), and the changes are within the normal range. Most of the electrolyte have no significant change(p > 0.05), only a fraction of electrolyte change obviously(p<0.05). In plasma group and whole blood group, we concluded that HB-H-6 resin untreated has consistent absorption efficiency on common composition(electrolytes, proteins, complement) between the whole blood and plasma. The second part of the experimental results show that: the staticexperiments have shown that white blood cells, red blood cells and platelets dropped by about 11.14%, 11.14% and 11.14% respectively in no prefilled group after 2h`s adsorption. The result of prefilled group is better than not prefilled group, white blood cells, red blood cells, platelets, dropped by about 5.1%, 1.8% and 5.1% respectively. Dynamic experiments have shown that in 30 min, 60 min and 120 min, the adsorption rate of white blood cells(1.5%, 3.2%, 6.82%), red blood cells(1.36%, 1.91%, 3.55%) and platelet(5.2%, 8%, 12.2%) in prefilled group is less than the adsorption rate of white blood cells of adsorption rate(3.3%, 7.8%, 11.5%), the red blood cells(2.2%, 5.1%, 7.7%),and platelet(28.2%, 44%, 55.2%) in no prefilled group. And At 30 min, 60 min and 120 min time point, the adsorption rate of white blood cells(1.85%, 1.25%, 0.98%), red blood cells(1.38%, 0.97%, 0.51%) and platelet(3.1%, 5.4%, 3%) in prefilled group is less than the adsorption rate white blood cells(3.04%, 6.2%, 2.7%), red blood cells(2.3%, 1.3%, 0.97%) and platelet(13.9%, 24.3%, 14.9%) in no prefilled group too. The third part of the experimental results show: the adsorption rate of TP, ALB, GLO, white blood cells, red blood cells, platelets were 5.83%, 10.54%,3.54%, 2.94%, 2.41% and 3.81% respectively in PDMS coated group. Among the three kinds of resin, the resin coated by PDMS has the best blood compatibility.Conclusion 1. The HB-H-6 resin without any process has the poorest blood compatibility. Although the adsorption in the common composition of whole blood and plasma has no difference, it has a serious damage in the formed elements in whole blood, and the damage to the blood platelet is particularly acute. 2. The blood compatibility of the prefilled group is better than the untreated group, and the operation of prefilled group is very simple and very suitable for industrial mass production. It is easy to implement relatively. 3. The blood compatibility of the PDMS coated group is better than the prefilled group, it is the best among the three kinds of resin. But its operation is relatively complex. It has the certain difficulty in industrial batch production. 4. The study has provided a certain theoretical basis to procceed the whole blood perfusion in clinic. But whether the whole blood perfusion can really replace the plasma perfusion combined with plasma exchange or not, still needs to be further researched. |
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