Study Of The Hydrogen Sulfide/Cystathionine -γ- Cleavage Enzyme System Change In The Fibroblasts On Burned Rats’ Wound

Objective To study the changes of Hydrogen Sulfide/Cystathionine-γ- Cleavage Enzyme System in the fibroblasts on burned rats’ wound.Method Adopt fibroblasts from burned rats as study object. To select 78 healthy SD rats and randomly divide into 13 groups(normal control group, normal saline group on 3rd day, 7th day, 14 th day, Na HS group on 3rdday, 7th day, 14 th day, PPG group on 3rd day, 7th day, 14 th day, Na HS+PPG group on 3rdday, 7th day, 14 th day), The normal control group received no intervention, the rats in rest of the groups were created 5% TBSA partial thickness burn wounds and take biopsy forconfirming, the rats in normal saline group were timely initiated intraperitoneal injection of salinedaily, the rats in Na HS group were timely initiated intraperitoneal injection of Na HS solutiondaily, the rats in PPG group were timely initiated intraperitoneal injection of propargyl glycine solution daily, the rats in Na HS+PPG group were timely initiated intraperitoneal injection of Na HS + propargyl glycine solution daily, to separate fibroblasts from the wound on the 3rd, 7thand 14 th day after burn and culture in vitro; to measure the concentration of hydrogen sulfide incell culture supernatant; the CSEm RNA gene relative expression level and CSE protein relative expression in fibroblasts.Results To compare the concentration of hydrogen sulfide at different time point in each group, there were significant difference between normal control and normal saline group(NS3, NS7, NS14)(P<0.05); and there were significant difference between Na HS group(N3, N7, N14) and normal saline group(NS3, NS7, NS14)(P<0.05); at the same timepoint, compare PPG group(P3, P7, P14) with the normal saline group(NS3, NS7, NS14), therewere not significant difference(P>0.05); compare Na HS group(N3, N7, N14) with Na HS + PPG group(N+P3, N+P7, N+P14), there were significant difference(P<0.05); compare the PPG group(P3, P7, P14) with the Na HS + PPG group(N+P3, N+P7, N+P14), there were not significant difference(P>0.05); to compare the intracellular CSEm RNA changes in each group,there were significant difference between saline group(NS3, NS7) and normal control group(P<0.05); and there were not significant difference between saline group NS14 and normalcontrol group(P>0.05), there were significant difference between Na HS group(N3, N7, N14)and the saline group(NS3, NS7, NS14)(P<0.05); there were significant difference between PPG group(P3, P7) and the saline group(NS3, NS7)(P<0.05), there were not significant difference between PPG group P14 and the saline group NS14(P>0.05); at the same timepoints, there were significant difference between Na HS group(N3, N7, N14) and Na HS+PPG group(N+P3, N+P7, N+P14)(P<0.05); there were significant difference between PPG group P3 and Na HS+PPG group N+P3(P<0.05); there were not significant difference between PPG group(P7, P14) and Na HS+PPG group(N+P7, N+P14)(P>0.05); compare the CSE protein synthesis during H2 S secretion process, there were significant difference between the saline group(NS3, NS7, NS14) and normal control groups(P<0.05); at the same time point, there were significant difference between Na HS group(N3, N7, N14) and the saline group(NS3, NS7, NS14)(P<0.05); there were not significant difference between PPG group(P3, P14) andthe saline group(NS3, NS)(P>0.05), there were significant difference between PPG group P7 and the saline group NS7(P<0.05), there were significant difference between Na HS group N3 and Na HS+PPG group N+P3(P<0.05), there were significant difference between Na HS group(N7, N14) and Na HS+PPG group(N+P7, N+P14)(P<0.05), there were not significant difference between Na HS group(N3) and Na HS+PPG group(N+P3)(P>0.05); there were significant difference between PPG group(P3, P7) and Na HS+PPG group(N+P3, N+P7)(P<0.05), there were not significant difference between PPG group(P14) and Na HS+PPG group(N+P14)(P<0.05).Conclusion The function of fibroblasts hydrogen sulfide/cystathionine-γ- cleavage enzyme system is reduced on burned rats’ wound. Exogenous hydrogen sulfidecould facilitate the function of fibroblasts hydrogen sulfide/cystathionine-γ- cleavage enzyme system. PGG inhibits the function of fibroblasts hydrogen sulfide / cystathionine-γ- cleavage enzyme system, therefore inhibits the produce of endogenous H2 S.