The Correlation Between Rats Of SAP With Hepatic Lesion And TNF-α At Different Altitudes

Objectives To establish rats with SAP model at high altitude area by animal experiments in the case of zero intervention and observe the expression of TNF-α in damaged liver at different times. This study aim to clarify the relationship between hepatic lesion and the expression of TNF-α from the perspective of cytokines in high altitude hypoxia.This study will provide the basis for the mechanism study of diagnosis and treatment of severe acute pancreatitis.Methods The experimental object is SPF grade male Wistar rats(the experimental animal center of Xi’an Jiao Tong University). The establishment of SAP rat model in Xi’an, Xining, Xinghai using Sodium taurocholate injection. Each altitude group was divided into sham operation group, 1H, 6h, 12 h, 24 h group.The content of the ALT、AST、AMY in serum will be measured by centrifugation. The next step is making tissue microarray by the method of Immunohistochemistry and mearsuring the content of TNF-α in liver..The content of the ALT、AST of SAP rat will be measured in different altitude and the correlation between the expression of TNF-α and altitude will be verified. The SPSS 19.0 statistical software will be used for data analysis.Results After SAP rats model is successful established, the expression of ALT, TNF- alpha, serum amylase, pancreatic case score and AST were increased in the same altitude group, and the value of P compared with each other was <0.05;In the same time group, the expression of ALT, TNF- alpha, serum amylase, pancreatic case score Is not related to the altitude change,the value of P is >0.05; 1H, 6h, 12 h, 24 h group, TNF-, ALT alpha expression, serum amylase, pancreatic case score, AST increased with altitude...