The Performance Analysis And The Exploration Of Biological Activity Of The PGS/PLLA Electrospuning Films Material

Purpose:The excellent biological activity of PGS and good mechanical properties of PLLA were spinning forming new nano fiber scaffold materials in the use of the electrostatic spinning technology. on purpose of looking for a kind of excellent tissue engineering scaffold material for the temporomandibular joint disc, the different proportion of fiber membrane was studied by means of physicochemical and biological properties.Materials and Methods: A novel electrospinning scaffold material of poly glycerolsebacate/ poly-l-lactic acid(PGS / PLLA) was prepared. The mass ratios of PGS and PLL A chosen were 1:3, 1:1, 3:1 and formulated into 25% of the spinning solution using hexa fluoroisopropanol(HFIP) as a solvent. Morphology, weight loss, and pH value changes in the PBS and the mechanical properties of the materials were measured during the PGS/PL LA electrospuning films degradation. TMJ disc cells were co-cultured with PGS/PLLA scaf fold and cell cytotoxicity and proliferation were determined by MTT methods. Hematoxyli n-eosin staining, safranin O staining and immunohistochemistry staining were tested at the14 day.Results: SEM indicated that electrospuning films showed uniform distribution and formed a vessel net. Added PLLA had little impact on the structure of PGS polymer. The crystallinity of electrospuning films increased with rising PGS level. TG indicated that PLLA improved the thermal stability of the PGS/PLLA electrospuning films. The contact water angle meter analysis indicated that the better hydrophilic property of the electrospuning films with the existence of PGS.The result of tensile tests suggested that the PGS/PLLA electrospuning films showed better Mechanical properties than pure PGS. PGS/PLLA electrospuning films showed proper degradation performance. Nontoxicity of scaffold and favorable cell proliferation were observed.Conclusion: Adhesion, proliferation and metabolism of temporomandibular joint disc cells on the PGS/PLLA scaffold are good. PGS/PLLA scaffold is expected as the temporomandibular joint disc tissue scaffold.