Preparation And Characterization Of Biodegradable Conductive Poly(3,4-ethylenedioxythiophene)/Poly(L-lactic Acid) Scaffold

In tissue engineering, we can apply electrical stimulation to control cell behavior in the tissue engineering scaffolds for tissue damage repair. Electrical stimulation for neural tissue repairment is remarkable. However, electrical stimulation needs conductive material to be the basement, the preparation of a good conductive scaffold is very necessary.Conductive HA (Hyaluronic Acid)-doped PEDOT (Poly3,4-ethylenedioxythiophene) particles were synthesized through chemical polymerization. To improve the conductivity of PEDOT particles, an orthogonal experiment was designed. The optimized conductivity measured by four-point probe is0.36s·cm-1, at EDOT0.01mol, dopant HA0.05g, oxidant APS(ammonium persulfate)0.015mol, reaction time24hour. Incorporating HA-doped PEDOT particles to biodegradable PLLA (Poly L, lactic acid), a conductive biodegradable PEDOT/PLLA composite membrane was prepared. FTIR and EDX confirmed the presence of HA in the PEDOT particles, the surface morphology of PEDOT particles and PEDOT/PLLA composite membrane were observed by SEM. About cytocompatibility, PCl2were cultured on the30%PEDOT/PLLA membrane treated by LN (laminin protein), the cells demonstrate normal morphology, high adhesion and cell synaptic longer attach on the membrane observed by metallographic microscope and fluorescence microscope. Compared with control group, PEDOT/PLLA composite membrane effectively increased the cell viability by116.6±3.2%after72hour determined by MTT. The results show that PEDOT/PLLA membrane has good cytocompatibility, it is conducive to PCl2cell synapse elongation and the formation of cell network structure. Conductive biodegradable PEDOT/PLLA membrane is significant in nervous tissue engineering to fabricate biodegradable conductive scaffolds.