Mechanism Of Baicalin In Inducing Apoptosis Of Human Gastric Cancer Cells

Objective: To investigate the proliferation inhibition of baicalin in human gastric cancer SGC-7901, BGC-823 and MGC-803 cells and to explore the possible molecular mechanisms of apoptosis induction.Methods: The proliferations of SGC-7901, BGC-823 and MGC-803 cells after treatment with 10, 20, 40, 80, 160 and 320μmol/L baicalin for 24, 48, 72 and 96 h were detected by MTT assay. After SGC-7901, BGC-823 and MGC-803 cells treatment with 80, 120 and 160μmol/L baicalin for 48 h, the apoptosis was detected by FCM.Array PCR was used to screen the key molecules in the apoptosis of gastric cancer cells induced by baicalin.The expression levels of factor associated suicide(FAS), factor associated suicide ligand(FASL), tumor necrosis factor related apoptosis inducing ligand(TRAIL), caspase 3 and caspase 8 mRNA and proteins were measured by real-time fluorescent quantitative PCR and Western blotting, respectively.Results: 10~160 μmol/L baicalin could suppresses the proliferation of SGC-7901, BGC-823 and MGC-803 cells in a concentration-and time-dependent manners(all p < 0.01).The results of FCMshowed80, 120 and 160μmol/L baicalin could induce the apoptosis of MGC-803 and BGC-823 cells in a concentration-dependent manner(all p < 0.01). The results of array PCR showed that the expression of the key molecules of death receptor pathway in the SGC-7901 cell induced by baicalin was significantly different from that in the control group(all p < 0.05).The expression levels of FAS, FASL, TRAIL, caspase 3 and caspase 8 mRNA and proteins in SGC-7901, BGC-823 and MGC-803 cells after treatment with 80, 120 and 160μmol/L baicalin were up-regulated(all p < 0.01).Conclusion: Baicalin can inhibit the proliferation of gastric cancer cells and induce cell apoptosis. This effect may be related to the death receptor pathway.