Effect Of Sinomenine On Levels Of Nuclear Factor Kappa B And Inducible Nitric Oxide Synthase During Renal Ischemia-reperfusion In Rats

Objective: To evaluate the effect of sinomenine on levels of nuclear factor kappa B(NF-κB) and inducible nitric oxide synthase(iNOS)during renal ischemia-reperfusion in rats.Methods: Seventy-two healthy male Wistar rats,weighing 180-220 g,were randomly assigned into 4 groups(18 each group) : sham operation group(group C), sham operation combine sinomenine group(group SIN),renal ischemia-reperfusion injury model group(group I/R),sinomenine pretreatment group(group SIN+I/R). The animals were anesthetized with 10% chloral hydrate 350 mg/kg.In group I/R and SIN+I/R,renal ischemia-reperfusion injury model were established,and the left renal pedicles were clipped with atraumatic microclips for 45 min followed by reperfusion;while in group C and SIN,renal pedicle were only exposed but not ligated for 45 min. In group SIN and SIN+I/R, sinomenine 60 mg/kg was injected intraperitoneally at the time of 30 min before reperfusion,while the equal volume of normal saline was given at the corresponding time point in group C and I/R. At the reperfusion time of 6h(T1),8h(T2),12h(T3),6 rats of each group were cut the left renal and killed after gathered the blood samples by cardiac puncture for measurement of serum creatinine(Cr) and blood urea nitrogen(BUN) concentrations. Pathological changes in renal tissues were observed with light and electron microscopes. The expression of NF-κB and iNOS in renal were determined by immune-histochemistry.Results: Compared with group C,the lever of serum Cr and BUN, expression of NF-κB and iNOS were no significant in group SIN(P﹥0.05);the lever of serum Cr and BUN,expression of NF-κB and iNOS were significantly increased in group I/R and SIN+I/R(P<0.05). Compared with group I/R,the lever of serum Cr and BUN,expression of NF-κB and iNOS were significantly lower(P<0.05),the microscopic examination showed that the renal pathological injury was obviously attenuated in group SIN+I/R.Conclusion: Sinomenine pretreatment can attenuate renal ischemia-reperfusion injury of rats and its mechanism may be related to inhibition of the activation of NF-κB and the expression of iNOS.