The Study On HPLC Fingerprint Analysis Of Pinelliae Rhizoma Praeparatum Dispensing Granules

Pinellia tuber which is perennial herb, is the dry tuber of Pinellia ternate(Thunb) Breit which is recorded in over years of ‘’Chinese Pharmacopeia”,belonging to Araceae.It’s warm in nature,acrid flavor and deleterousness,belonging to the main and collateral channels of the spleen channel,stomach meridian and lung channel. Pinellia tuber which is an important Chinese herbal medicine is mainly used for the treatment of eliminating dampness and resolving phlegm, preventing or arresting vomiting,removing blood stasis. Because of its poisonousness, it is mostly processed before it is ready for clinical application. Pinellinae Rhizoma Praeparatum is mixed with licorice and lime as for materials processing, which is common Chinese medicine herb recorded as one of processed products of Pinellia tuber in “Chinese Pharmacopeia”2015Edition. Pinellinae Rhizoma Praeparatum is mainly used for the treatment of eliminating dampness and resolving phlegm, preventing or arresting vomiting,removing blood stasis. Pinellinae Rhizoma Praeparatum dispensing granules which is made of conform the processing standards of pinellia tuber pieces after extraction, concentration, drying and granulating process, is a new form pieces.It has broken classic mode that Chinese medicine needs to decocting method, having characteristic that it is easy to use without water decocting method, to store, to carry and so on. Because of Chinese medicine dispensing granules have distinguish appearance of its original herb, lost its specific “characeter” and “microscopic identification”, to establish the standard of Pinellinae Rhizoma Praeparatum dispensing granules is obvious significant. It is the key link to be sure of quality of product.Chapter1 The study on the status of Pinellinae Rhizoma Praeparatum dispensing granulesThis chapter is to review the study at home and abroad of Pinellinae Rhizoma Praeparatum dispensing granules and its herb, it is mainly consist of study on herbal origin, study on chemical aomposition, study on pharmacological action and study on quality standard. According to the research we knows that the Pinellinae Rhizoma Praeparatum dispensing granules has lots of pharmacological effects, including that can relieve a cough, anti-inflammatory, antioxidant and so on. In addition to anti tumor, vomiting, anti early pregnancy, anti-ulcer that Pinellia tuber has its essential effect. There are more research on the chemical composition and pharmacological action of the herb at home and abroad, Research on the Pinellinae Rhizoma Praeparatum dispensing granules is blank at present. Because of its characters,it can quite be adapt to the fast pace of modern life.It is obvious a starting point that it can make traditional Chinese medicine be modernization and internationalization. So Making deep researches in aspect of dispensing granules seems that it is very urgent.Chapter2 The study on quality standard of Pinellinae Rhizoma Praeparatum dispensing granulesThe study on quality standard of Pinellinae Rhizoma Praeparatum dispensing granules is including the following aspects:1.The test of character identification of Pinellia granule: Through the observation of the 10 batch of Pinellia tuber of granule shape and appearance, summed up its appearance results as basis of quality standard of Pinellia granule character identification.2. The test of P physicochemical identification of inellia granule: The experiment is based on Chinese Pharmacopoeia 2015 Edition. By checking the size, moisture, solubility, extract, TLC and recording experimental data, it can be sure the methods and standards for kinds of identify inspection, which could provide the basis for addition of physicochemical quality standard of the Pinellia granules.3. The test of standard of check items of Pinellinae Rhizoma Praeparatum dispensing granule: It measures the micro organism, pesticide residue, heavy metal ect by commonly used methods to be sure of the methods and the standards. it filled in the blanks of check items of the quality standard of Pinellinae Rhizoma Praeparatum dispensing granule.4. The test of determination of the content of effective components: Both ammonium glycyrrhetate and ephedrine hydrochloride are the main components of Pinellinae Rhizoma Praeparatum dispensing granule, the amount of them is great significance to standard of Pinellinae Rhizoma Praeparatum dispensing granule. The amount of ammonium glycyrrhetate and ephedrine hydrochloride of 10 batches of Pinellinae Rhizoma Praeparatum dispensing granules are tested by using HPLC and UV spectrophotometry. The results showed that the amount of ammonium glycyrrhetate is not less than0.02% and the amount of ephedrine hydrochlorideis not less than0.01%, it has set the standard of them according to result.Chapter3 The study on the HPLC fingerprint of Pinellinae Rhizoma Praeparatum dispensing granuleStudy on fingerprint of traditional Chinese medicine granules is a hotspot in recent years. The HPLC fingerprint of Pinellinae Rhizoma Praeparatum dispensing granule are systematic researched in this experiment. It established the feature HPLC fingerprint of Pinellinae Rhizoma Praeparatum dispensing granule, according to the assaying result of 10 batches of Pinellinae Rhizoma Praeparatum dispensing granule. It is a new method to test content of Pinellinae Rhizoma Praeparatum dispensing granule.Chapter4 Summary and DiscussionAccording to the experiments in former, it could set up the quality standard draft of Pinellinae Rhizoma Praeparatum dispensing granule. The final part is summary and discussion based on the experiments.