Optimization Of Detection Methods For Three Common Drug

The upward trend of drug-cases has been spread around the world. Long-term drug taking will destruct one’s body, cause character twisting, and induce to self-injury, self-maltilation and commit suicide, lead to families broken, even to the leaving of wife and children. In court cases, we found the increasing of "drug driving" cases. In Northeast China, take "ice", "Magu" and "K powder" be the majority, and most drug users will taking these 3-types of drugs at same time to get a special pleasure.In this paper, the common drugs(i.e methamphetamine, caffeine, and ketamine) in Northeast China course cases were used for research subjects, to study the method for extract and detect these three kinds of drugs in human urine, blood and hair at one time. Pre-treatment methods and GC, GC / MS, HPLC instrument testing conditions were optimized, and the accurity and reliability of the methods were assessmented.Pretreatment of urine and blood: 0.2mol / L sodium hydroxide solution was used to adjusted pH 12, 3mL chloroform was used as organic solvent to liquid-liquid extraction alone with ultrasonic treated twice, 10 minutes each time, combined organic phases were evaporated then soluted in 100μL methol, use 1μL for instrumental analysis. Pre-treatment of hair: the hair was hydrolysed by 0.2mol/L hydrochloric acid; the hydrolysis condition optimized by orthogonal experiments wwre temperature 90 ℃, hydrolysis time 3h, PH> 11, the recovery rate of the three drugs up to 98%. Hydrolyzed hair was adjusted to pH 12 with 0.2mol / L sodium hydroxide solution, 3mL chloroform was used as organic solvent to liquid-liquid extraction alone with ultrasonic treated twice, 10 minutes each time, combined organic phases were evaporated then soluted in 100μL methol, use 1μL for instrumental analysis.GC condition was that HP-5 selected for column, FID detector, shorter programmed heating time; GC / MS condition was HP-5MS column, shorter programmed heating time; HPLC selected ODS column, using methanol and 0.3mol/L phosphoric acid buffer for mobile phase and 30:70 isocratic elution to separate analytes. Linear coefficient of urine, blood and hair drugs in different concentrations and different detection equipment was greater than 0.99, linearity fit well. Inner and intra-day recovery at different concentration levels and testing instruments are controlled between 89.45%-119.55%; accuracy intra-day RSD and inner day RSD values were less than 5. Limit of detection LOD between 1.05-2.87 ng / mL, the limit of quantitation LOQ between 1.35-3.01 ng / mL.The optimized method for drugs detecting is more accurate, faster, more simple, easier to operate and widely used. The requirements for laboratory reagents and equipment of this assessment is simple, it can verify whether drug addicts taking drugs quickly. It provided a strong support for rapid case-solving and compulsory detoxification and other narcotics work. It can meet the requirements of the criminal technical work for the grassroots units.