Natural Macromolecular Composite With Hydrophilic PVA As Scaffold For Tissue Engineering

Objective: to prepare a composite film material with blending polyvinyl alcohol(PVA), hyaluronic acid(HA) and alginate(Na Alg) and to investigate the feasibility of serving as a tissue engineering scaffold for the purpose of tissue repairing.Methods: first, blend PVA of different relative molecular weight with different viscosity Na Alg, then the mixture crosslinked by Ca Cl2 and formed PVA-Alg Ca2+film material. Second, Select the best of the PVA-Alg Ca2+ film,and add HA with different content and different relative molecular into the film, then measure its mechanical properties and observe its tissue morphology with scanning electron microscope, discusses the influence of different factors on the properties of materials.Results: all of the PVA-Alg Ca2+ thin film material, moisture content is between53.4% and 87.3%, within the range from 128% to 438% inflation rate, the value of the porosity in 55.9% to 89.4% range. After adding HA, the maximum moisture content of PVA- Alg Ca2+-HA film increase to 91.4%, expansion ratio and porosity has no obvious change. The observation of the material inner structure through scanning electron microscopy proved that the film internal organization is porous structure similar to foam.Conclusion: PVA-Alg Ca2+-HA thin film material has high moisture content and ideal porous structure, its mechanical performance is good, so it is suitable for use as tissue engineering scaffolds.