Exploring The Influence Of QingYi â…¡ On The Intestinal Biological Barrier In Rats With SAP And The Mechanism

Objective: Establishing the models of severe acute pancreatitis in rsts, detectiving the level of serum TNF-α, activity of XO、SOD in intestine,the contraction of intestinal smooth muscles and the quantity of bifidobacterium、escherichia coli in faeces; and the changes of indexes mentioned above after using qingyi-II. Exploring the role of TNF-α and ROS in the chang of enterobacteria and the protection of qingyi-II, therefore providing theory basis for the qingyi-II in the treatment of acute pancreatitis.Methods: The body weights in 200g-300 g healthy SD rats(male or female) 40 were randomly divided into three groups:sham operation group(A group,n=8), SAP model group(B group, n=16), and Qing Yi II(QY-II)-treated group(C groun=16). The sham operation group were only flipped the intestinal canal; the SAP model group were induced by retrograde injection of 5% sodium taurocholate into bilio-pancreatic duct( 0.1ml/100 g, q6h); to fill the stomach with QY-II( 1ml/100g) to establish the QY-II-treated group. All rats in B、C groups were sacrificed after SAP had been induced at 12 hours and 24 hours after anesthesia.Observing the chang of pancreatic tissue and status of ascites; serumamy TNF-α was measured by ELISA; the activitiy of SOD in intestine was detected by WST-1 method、the activity of XO was detected by colorimetric method; using real-time polymerase chain reaction(RT-PCR)to detect the enterobacteria(escherichia coli、bifidobacterium). All test results be analyzed by statistical analysis SPSS17.0 software.Results: 1. Pancreatic tissues turned up congestion、edema immediately after making model of SAP. When opened the enterocoelia after 12 h 、24h, we found pancreatic tissues edema obviously,coupled with massive bleeding and hemorrhagic ascites, which meaned the suncessful establishment of SAP. 2. 12 h and 24 h after the establishment of SAP, the level of Serum TNF-α, the activity of XO in intestine, the number of escherichia coli andbifidobacterium were increased. however, the activity of ileal SOD, the contraction of intestinal smooth muscles, the quantity of bifidobacterium were decreased(P<0.05) compared with rats in sham group. Results in Qing Yi II-group showed up opposite tendency compared with SAP group 3. When compared with the 12h-group,the activity of XO in intestine, the number of escherichia coli in rats of 24h-group in B、C group were increased. The contraction of intestinal smooth muscles, the level of Serum TNF-α, the activity of ileal SOD, the quantity of bifidobacterium were decreased(P<0.05). The rats in Qing Yi II-group had contrary results.The contraction of intestinal smooth muscles, the activity of ileal SOD, the quantity of bifidobacterium of rats in 24h-group were increased(P<0.05), the level of Serum TNF-α, the activity of XO in intestine, the number of escherichia coli were decreased(P<0.05).Conclusion: Qingyi-II has the function of ameliorating inflammatory responseoxidative stress response in SAP, protecting the microflora dysfunction in gut, thereby protect intestinal biological barrie.