The Effect Of Fullerenol Combined With Cisplatin On The Proliferation Of Cervical Cancer HeLa Cells

Objective: To investigate the effect of fullerenol combined with cisplatin on the proliferation of cervical cancer He La cells in order to provide new ideas and laboratory theoretical basis for the clinical treatment of cervical cancer.Methods: Cervical cancer cell line He La cells in vitro were treated with different concentrations of fullerenol, different concentrations of cisplatin, and different concentrations of fullerenol combined with cisplatin, after24 h,48h,72 h, invert microscope was used to observe the changes and quantity of cell appearance and to take photos; MTT assay was used to determine the effect of drugs on the proliferation of He La cells. Flowcytometry technology(FCM)was used to analyze the changes of cell apoptotic rate of Hela cells treated with different concentrations of fullerenol combined with cisplatin for 24 h.Results:(1)Cell changes were observed in an inverted microscope, the combination group shows cells became round and the distance between them increased, a large number of apoptotic cells in suspension within the field of vision. Fullerenols groups and cisplatin groups with different concentrations have inhibitory effect on the He La cells in a dose-dependent and time-dependent way when they are used alone( P<0.05);Compared with cisplatin alone, inhibition rate of different concentrations of fullerenols(5.0ug / ml and 10.0ug/ml) combined with cisplatin(5ug/ml) increased significantly(P <0.05). Flow cytometry analyzed that combined group could induce Hela cells apoptosis,the apoptosis rate of fullerenols combined with cisplatin groups were higher than cisplatin group(P<0.05) while the concentrations of fullerenols was 2.5ug/ml.Conclusions: The inhibition of fullerenol combined with cisplatin on He La cells in vitro is more significant, resulting in a stronger anti-cancer effect.