Studies On Evodia Rutaecarpa And A Plant Endophytic Fungi Of Tripterygium Wilfordii Metabolite Of Activive Constituents

This paper was designed to research chemical compositions of Evodia rutaecarp-a and a strain of endophophyic fungi(Talaromyces wortamnii LGT-4) secondary met-abolites. Twenty-four compounds were isolated and purificated from Evodia rutaecar-pa and Talaromyces wortamnii LGT–4 by silica gel column chromatogramphy, MCI columnchromatography, Sephadex(LH-20), HPLC and recrystallization etc. The stuct-ures of 16 compounds have been elucidated. Structural elucidation of compounds we-re based on physicochemical properties and various spectral data analysis(1H-NMR,13C-NMR etal). The anti-acetylcholinesterase(AchE) and anti-monoamine oxidasone(MAO) activity of these compounds were detected by using 96-well microplate metho-ds. The main results were as following:1. Ten compounds obtained from Evodia rutaecarpa(Juss.) Benth. Seven of the-m were identified as Limonoid(1), WuchuyuamideⅠ(2), Evodiamine(3), Rutaecarpin(4), Dievodiamine(5), Evodol(6), Evodiagenine(7). Compounds 3 and 5 showed MAO inhibitory activities with IC50 values of 55μg/mL, 68μg/mL, respectively. The AChE inhibitory activity of these compounds showed no inhibitory activity aginst AChE.2. Forteen compounds isolated from the(CYM) fermentation broth of Talaromy-ces wortamnii LGT-4 and some of them were identified as 4-hydroxyphthalide(8), 1,3,4,5,6,7-hexahydro-6,7-dihydroxy-7-methyl-3-(1-propenyl)-8H-2-Benzopyran-8-one(9),Ergosterol(10),3-(2-hydroxypropyl)-8-hydroxy-3,4-dihydroisocoumarin(11),Ciscy cl-o(L-Ala-L-Pro)(12),6-Amino-3-(4-hydroxybenzyl)-1,4-diazonane-2,5-dione(13),As-pergillumarin B(14), Deacetylisowortmin B(15), Entonaemin A(16). The AChE and MAO inhibitory activity of these compounds were determined by 96-well microplate methods.The result showed that compound 8, 9, 12 have MAO inhibitory activity with IC50 values of 35μg/m L, 27μg/mL, 28μg/mL, respectively.