Active Components Of Comprehensive Utilization Of Snake Venom

Nowadays, the diseases of blood coagulation system have been concerned for their mortality and morbidity. There are more than five million patients who were suffering for this kind of diseases every year in China. Many a pharmaceuticals are required to be researched and developed. Compared with congener products, venom, as a kind of biological preparation, has lots of advantages such as high efficiency, fewer by-effects, and secure usage, and would be extensive applied in clinic. However, the researches about the utilization of venom enzymes are most focused on a single enzyme. It would waste many biological resources. Therefore it is necessary that as many as possible enzymes should be isolated from the same venom preparation.Snake venom was fractionated by chromatographies. The thrombin-like enzyme and blood coagulation factor X activator were subjected to SDS-PAGE. The result of experiments shows that the enzymes are single bands on SDS-PAGE. The molecular mass of thrombin-like enzyme is 36.1kD, and that of blood coagulation factor X activator is 41.4kD. The isoelectric point of thrombin-like enzyme and blood coagulation factor X activator is 4.42 and 4.00 determined by isoelectric focusing electrophoresis respectively. The result of glycoprotein staining showed that they are glycoproteins.This article describes a simple method to isolate thrombin-like enzyme and blood coagulation factor X activator from the same venom preparation. The method we describe here is an efficient approach for the comprehensive utilization of the venom.