| The purpose of this subject research is to study the mechanism of YinqiaoDetoxication Oral Liquid in anti-influenza virus. Immunodeficient micewere used as animal models in the study and infected influenza virus FM1nasally. Then the immunological characteristics in the mice were observedafter the treatment ofYinqiao Detoxication Oral Liquid.Immunological mechanism of anti-influenza virus of YinqiaoDetoxication Oral Liquid(YQD) in SCID mice was described in SectionOne in this papers. All the mice, except control group, were infected withinfluenza virus A/FM/1/47-MA(FM1) intranasally with 15 ofTCID50(Tissue Culture Infective Dose). Then the mice were administratedwith physiological saline, 0.07 g?kg-1 of ribavirin or 5.0 g?kg-1, 10.0 g?kg-1,20.0 g?kg-1of YQD, respectively. After infection of 1 day, 3 days, 5 days,7days, repectively, spleen and serum were collected. NK cell lysis assayand expression of CD3+CD4+ T cell were detected respectively inspleenocytes. Contents of IFN-γ, TGF-β1 and TNF-αin serum were meassured by ELISAassay.After curation by YQD, NK cell lysis and the amount of CD3+CD4+ Tcell in spleenocytes and the levels of IFN-γand TGF-β1 in serum were increased. Serum concentration of TNF-αdecreased in YQD treatment groups. Contents of IFN-γreached the peak value on the 3th day and continued to the 5th day. Later, the level of IFN-γretured to normal level. The variation tendency of NK cell activity in spleen was in accord with thatof IFN-γ. But it didn’t reach the maxium value until the 5th day. The activity of NK celll in three groups of YQD was well above that of model group. In addition, therapeutic action of both 10.0g?kg-1 and 20.0g?kg-1 ofYQD treatment groups were better than YQD5.0g?kg-1. Contents ofTGF-β1 became higher on the 3th day than the 1st day and reached the peak value on the 5th day. Later, the activity of TGF-β1 retured to the normal level. The level of TNF-αdecreased during the 7-day period. In addition, therapeutic action of both 10.0g?kg-1 and 20.0g?kg-1 of YQDtreatment groups were better thanYQD5.0g?kg-1.The immune function in SCID mice was improved by YQD, thus, wesurmised that the capability of antivirus in mice increased indirectly.The immunological mechanism of anti-influenza virus of YQD inSCID/Beige mice was represent in Section Two in the papers. All the mice,except control group, were infected with influenza virus FM1 intranasallywith 15 of TCID. Then the mice were administrated with physiologicalsaline, 0.07 g?kg-1 of ribavarin or 5.0 g?kg-1, 10.0 g?kg-1, 20.0 g?kg-1 ofYQD, respectively. After infection of 1 day, 3 days, 5 days, 7days,repectively, spleen and serum were collected. NK cell lysis assay,expression of CD3+CD4+ and CD3+CD8+ T cell subsets were detectedrespectively on spleenocytes. Contents of IFN-γ, TGF-β1 and TNF-αin serum were meassured by ELISAassay.After the treatment of YQD, NK cell lysis, amount of CD3+CD4+ andCD3+CD8+ T cells in spleenocytes increased. Serum concentration of TNF-αdecreased at first 5 days and then increased on the 7th day in all YQDtreatment groups. The concentration of TGF-β1 in serum increased at first 3 days and decreased from the 5th day in YQD treatment groups. SerumIFN-γwas not detected.The immune function in SCID/Beige mice was improved by YQD, suchas NK cell lysis and amounts of T lymphocytes in spleen and contents ofTNF-αand TGF-β1 in serum. Thus, we speculated that the capability of antivirus in mice increased indirectly via the treatment ofYQD.In conclusion, both specifical and non-specifical immune responses were improved by YQD. Moreover, influenza viral load in lung decreased afterthe treatment of YQD. So we demonstrated that YQD could play direct andindirect roles in anti-influenza virus. |
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