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The Effect Of High/Low Endurance Exercise On Sirt3 And SESNS Relevant Antioxidant Gene In Quadriceps Femoris Muscle Of SAMP6 Mice

Posted on:2017-05-20Degree:MasterType:Thesis
Country:ChinaCandidate:B B ChenFull Text:PDF
GTID:2297330485968938Subject:Human Movement Science
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The basic move of mankind is the contraction of muscle, skeletal muscle is the foundation of relevant daily work and life of human. The quality and strength of human decrease during aging. Modern society, with the gradual increase of human population, the healthy condition of old people attracts most of human attention, sarcopenia or Attenuation of the skeletal muscle syndrome is a important question that impacts the health of mankind. This condition of aging cause the fall or fracture of older people and consequent it will increase the burden of themselves> their family and society. Mitochondria dysfunction is the major reason of sarcopenia, the entire mitochondria dysfunction happened during skeletal muscle aging, it consists of decreasing mitochondria biogenesis, enhanced oxidative stress of mitochondria and the efficiency of generating ATP declines, oxidative stress of mitochondria plays a crucial role in the mitochondria dysfunction, This main source of reactive oxygen species is the mitochondria, the continuous increase of reactive oxygen species and the loss of antioxidant enzyme make the oxidative stress occurs. The increasing oxidative stress in skeletal muscle cell result in muscle fiber apoptosis, disturbing the balance of protein metabolism, which may be the important mechanism that leads to sarcopenia.Objective:The aim of the research is to explore the effect of relevant antioxidative genes of sirt3 and sestrins during oxidative stress of quadriceps femoris muscle.We treat higher/lower intensity endurance training as investigate measure. Choosing old SAMP6 strain senescence accelerated mice and 4 months mice as model, we investigate the function of different intensity enduring training in quadriceps femoris muscle of SAMP6 mice. It will contribute to relief relate symptoms of Sarcopenia and develop the relevant theory.Methods:it was stochastic that the mice divided into four group:accelerated control group(P), higher intensity group(H) and lower intensity group(L). Killed by cervical and dissected from quadriceps femoris muscle. Then the relevant researchers (mainly author) measured the mRNA expression level of antioxidative gene Sirt3、PGC-1α、P53、Sesn1、Sesn2、in quadriceps femoris muscle by Real-Time PCR and the level of protein associate antioxidative gene Sirt3、 PGC-1α、P53、Sesn1、Sesn2 through western blotting.Results:The expression levels of protein and mRNA of Sirt3 between endurance training and control group have no significant difference, although there is an increasing tendency, there is a marked difference between L group and P group (p<0.05) in the protein expression levels of PGC-la, however, the mRNA expression levels of PGC-lais higher than R group and lower than H group(p<0.05). The L group expression levels of P53 protein is increased significantly as compared to R group (p<0.05). There is a marked difference between H group and L group (p<0.05) in the expression levels of SesnlmRNA, and the H group increased significantly as compared to L group (p< 0.05), at the same time, the expression levels of Sesnl protein in R group is significantly lower than the L group (p<0.05). Sesn2 activities in H group was increased in L group and P group (p<0.05), in addition, there is a marked difference in L group and P group(p<0.05), the expression levels of Sesn2 protein in P group is significantly lower as compared to the L group (p<0.05)Conclusion:Endurance exercise regulates organism’s metabolism and redox condition and so on. The higher intensity endurance training increased the expression levels of protein of the SESN2 because that higher intensity endurance training generates higher anaerobic condition. The higher intensity endurance training declines the activity of PGC-la mRNA probably comes from the transient activity of PGC-1α mRNA. Although exercise generate a little of adapting change to the expression levels of protein of Sirt3、PGC-1α、P53、Sesn1、Sesn2, the expression levels of and protein of Sirt3、PGC-1α、P53、Sesn1、Sesn2 is not accordant, maybe it relevant other pathways that higher intensity enduring exercise and lower intensity enduring exercise.
Keywords/Search Tags:ROS, endurance training, oxidative stress, skeletal muscle
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