An Experimental Study On Characteristics Of Methanogenesis By Hydrolysis Of Organic Organic Compounds In Coals

There is rich concentrations of methane in the coal bearing and adjacent strata in the Huaibei Plain.It is famous region which contains high gas with deep exploitation potential.It has been found a number of Methane Production Bacteria(MPB)in groundwater of deep mines by bacterial gene sequencing techniques.The experimental researchs on methanogenesis are carried out with groundwater samples as a source of the bacteria From Renlou Coal Mine under ground hole(final hole horizon:C31-3carboniferous Taiyuan Formation containing small cave lime rock).It shows that there are small molecular organic compounds by degradation of macromolecular organic matter.The trend of the concentration of propionic acid,ethyl acetate and ethyl caprylate increasing and then decreasing after the period of time.The results indicate the microbia flora in groundwater of Huaibei coalfeilds could conduct methanogenesis by utilization of macromolecular organic compounds.The compounds including 2-hexyl-1-Decanol,Bis(2-ethylhexy1)adpiate and Linoleic acid are degraded to product methane.Experimental results verify the validity of this algorithm,and displays a basic way of the hydrolysis of macromolecular organic matter extracted from coals.It has been found Methanoregula,Methanobacterium,Methanomethylovorans,Methanoregula,Methanolobus,Methanomassiliioccus in coal measures groundwater in Huaibei coalfeild by extraction of archaeal genome DNA,amplification of mcrA gene fragments and cDNA library and comparison of gene sequence homology.It explains there are abundant in species of MPB,Methanomassiliicoccus is discovered to fill the record blank in research area on MPB.