Endophytic Bacteria Diversity Of Abietinella Abietina In Two Diffrent Habitats

Abietinella abietina,dominant spices of spruce forest floors in Helan Nature Reserve and Baiyinaobao Nature Reserve,plays an important role in material cycling,conserving water and maintaining soils.Based on good growing environment supporting by A.abietina characteristic life form,bryophyte endophytic bacteria can have a positive effect in terms of host plant growth,development and forest ground layer ecosystem.In this study,we used Illumina-based 16SrDNA V5-V7 hypervariable regions sequencing to investigate endophytic bacteria living in A.abietina sampled from two different habitats and explore the relationship between bryophyte endophytic bacteria and substrate soil bacteria.The main results were as follows:1.We identified 14 pylum,29class,66 order,134 family and 189 genus from the resulting 145530 high quality sequences and 1170 Operational Taxonomic Units in Picea crassifolia forest A.abietina.The most dominant phylum in samples were Proteobacteria and Actinobacteria.Alphaproteobacteria and Betaproteobacteria were the dominant class;Bacteria assigned to Bacillales and Methylophilales were the dominant order;Sphingomonadaceae,Methylophilaceae,Comamonadaceae were the dominant family and the most dominant genus was Sphingomonas.Similarity coefficient analysis showed that the composition of bacteria between Abietinella abietina and substrate soils in P.crassifolia forest were similar at moderate level,74.19%endophytic bacteria OTUs could be found in moss surrounding soils.2.22 different pylum,41 class,78 order,150 family and 225 genus in Picea mongolica forest A.abietina were obtained from 137687 high quality sequences and 842 OTUs.Bacteria assigned to Proteobacteria and Firmicutes were the dominant phylum;Alphaproteobacteria,Betaproteobacteria and Bacilli were the dominant class;Bacillales,Rhizobiales and Sphingomonadales were the dominant order;Bacillaceae and Rhizobiaceae were the dominant family;Bacillus and Sphingomonas were the dominant genus.The results indicated that the percentage of OTUs shared between A.abietina endophytic bacteria and substrate soils bacteria communities were 50.59%.3.Based on Anosim and MRPP analysis,there were significantly difference in diversity of endophytic bacteria in two habitats.A.abietina endophytic bacteria diversity were much higher in P.crassifolia forest compared to P.mongolica forest.A.abietina in P.crassifolia forest owned 705 unique OTUs and the bacteria assigned to Betaproteobacteria,Sphingomonadales and Sphingomonadaceae was remarkably higher than A.abietina in P.mongolica forest.A.abietina in P.mongolica forest owned 377 unique OTUs and relative abundance of Microbacteriaceae were remarkably higher than former one.These results revealed that niche played important roles in endophytic bacteria diversity of A.abietina.In addition,465 OTUs were shared by two samples and some of them were not detected in substrate soils,revealed that the phylogeny of hosts might portray some influence on the associated bacterial community.