Hydrothermal Preparation Of Titania-Carbon Nanocomposite And Its Application As Electrochemical And Photoelectrochemical Biosensor Scaffold

With the rapid development of nanomaterials, the development of the biosensors has entered into a new stage. Nanomaterials with good physical, chemical, mechanical, electrocatalytic, photoelectrochemical property, biocompatibility and large surface area, etc., is a hot research spot currently. Application of novel nanomaterials on biosensor construction can effectively improve the sensitivity, wide the linear range and lower detection limit of the biosensors. This thesis mainly focuses on synthesizing titania composite nanomaterials with different morphologies by hydrothermal method, and applying them to prepare electrochemical enzyme sensors and photoelectrochemical biosensor with goog performance. The main contents are described as follows:1. Preparation of MWCNT-TNT nanocomposite and application of it as scaffold for HRP electrochemical biosensorTitania nanotubes (TNT) and its composites MWCNT-TNT were synthesized successfully by hydrothermal method, and the nanomaterials were characterized by transmission electron microscopy, X-ray diffraction and Fourier transform infrared spectroscopy. Then, HRP sensor was prepared using the nanocomposite as the carrier, Nafion-as a binder-to immobelize-HRP on the-glassy carbon-electrode.This biosensor was studied by electrochemical methods and finally applied to the detection of hydrogen peroxide.2. Preparation of GNP-TNT nanocomposite as Tyr electrochemical biosensor scaffoldSimilarly GNP -TNT composites were successfully synthesized by hydrothermal method and the nanocomposite was characterized using the same methods as above. Then, HRP electrochemical sensor was prepared using the nanocomposite as the scaffold, and evaluated for the detection of catechol.3. Preparation and application of MWCNT-TNP composite as scaffold for the LDH photoelectrochemical biosensorMWCNT-TNP nanocomposite was prepared by hydrothermal method, and the same techniques as above were used for characterizing the nanocomposite. Then, LDH photoelectrochemical sensor was developed using the nanocomposite to immobilize LDH and NAD+ on ITO electrode with the aid of EDC and NHS as crosslinking agent. Finally, the photoelectrochemical behaviour and analytical performance of the PEC biosensor was investigated by current-time curve method.