The Research Of Phenolic Compounds' Electrochemical Sensing Application Based On Aminated Glassy Carbon Electrode

The original amine neurotransmitters and the dihydroxybenzene isomers can be considered as phenolic compound because both of them have a hydroxyl group (-OH) which directly connected with aromatic nucleus. With the development of science and the improvement of medical technology, the orgin and the mechanism of some diseases are discovered. Scientists have founded that how the physiological active substances effect human body health, especially the neurotransmitter. Because the neurotransmitter in the central nervous system neurons play an important role in communication. Some neurotransmitter have important instruction function for the body's physiological and pathological process, such as dopamine(DA), acetylcholine(ACh), serotonin(5-HT), epinephrine(EP), etc. So developing a fast, reliable detection method is of practical importantance. Dihydroxybenzene, including hydroquinone (HQ),catechol(CC) and resorcinol(RC), as an important fundamental synthetic chemical compound, has been widely used to produce dyes, pesticides, developer, ect. However, these compounds are highly toxic to animals and can be absorbed by the skin causing poisoning symptoms, such as various neurological symptoms, chromosome aberration, tinnitus, vomiting. Besides, the compounds are difficult to be degraded, which pose great threat to the environment. Therefore, the determination of dihydroxybenzene isomers is very important.In this paper, we combined electrochemical methods with chemical modified electrode methods and developed a simple way to decect dihydroxybenzene and neurotransmitter as follows:1. The surface of galssy carbon electrode (GCE) was introduced nitrogen groups by electrolysis in ammonium carbamate solution. The dihydroxybenzene isomers played excellent electrocatalytic activity on modified GCE, which involving a two protons and electrons process. The oxidation peak of the isomers were separated perfectly, resulting in the liner measurement of one component. Besides, the modified GCE was applied to the detection of real samle and get a credible recovery. After miniaturization, the modified GCE was expected to apply to the field of samle test outside.2. The modified electrode was applied to detect EP and 5-HT. The modified GCE showed good response on both. And at the same time we measured the liner range and interference of EP and 5-HT with the common physiological active substances, such as AA and DA, which did not effect the result and got a liner range themselves. The electrode was expected to become a real-time on-line detector of human neurotransmitter...