Extraction Of Biological Active Components From Moringa Tree By Sub-critical Fluid

Moringa tree, native to northern India, has economic value. Oil content of Moringa seed is high and the seed coat can be used for water filter. Moringa leaf, one of health foods has effective on the prevention and treatment of many diseases such as hypertension and diabetes. Little results can be searched on further processing of Moringa tree, most of them focused on traditional techniques. In this paper, Moringa seed and Moringa leaf are used as raw material to produce ben oil and extract of Moringa leaf, using sub-critical fluid extraction technology, and then purifying extract of Moringa leaf by macroporous resin column chromatography.The seeds,which were out of shell,are smashed.And then ben oil are extracted using a sub-critical extraction kettle. The optimal conditions are determined by the single factor experiment, regarding the yield of ben oil as the target. The result shows that extraction temperature is 48℃, solvent burden ratio is 0.028 L/g and extraction 4 times. Compared with oil content measured by Soxhlet extraction, extraction ratio of sub-critical extraction is 99.37%. Then the physical and chemical properties, fatty acid composition and fatty acid positional distribution of ben oil are measured. 83.329% of all the fatty acid is unsaturated fatty acid, and most of unsaturated fatty acid is oleic acid which is distributed on sn-1,3 and sn-2. Besides, fatty acid with odd carbon number is found which provided the evidence that the ben oil has health value.According to the principle of sub-critical extraction, the sub-critical ethanol were used to extract Moringa leaf and increase the extraction ratio effectively. The optimal conditions were determined by the RSM experiment. When ethanol concentration is 70%, extraction temperature is 130℃ and extraction time is 2 hour, the result is that the yield of the content of flavone is 2.609%. Considering active polyphenolic properties, the vitro antioxidative was studied.The flavone was purified by macroporous resin column chromatography, using D101 macroporous resin. For the experiment, Eluant eluted in 1-5.5h were collecte Td. The result shows that the purity of flavone is 60.2%, and the yield is 46.3%, which is 4.3 times purer than the extraction by sub-critical and meet the requirements required.The continuous distillation process was simulated by Aspen Plus, separating the ethanol-water system. For distillation column, the stage number was set to 17, the mass reflux ratio was 0.8, the feed stage of raw materials was 9, and D/F was 0.71. Under above-mentioned conditions, the purity of ethanol is 77.4%, the recovery rate is 99.99%.