Effects Of Different Edible Oils Intake On Intestinal Fatty Acid Composition And Immun Barrier Function In Mice

Trans Fatty Acid(TFA)is a general definition for all unsaturated fatty acids containing trans-double bonds.The natural TFA is mainly derived from rumen microbial metabolism of ruminant intake of food.The TFA produced by food processing is mainly derived from isomerization of unsaturated fatty acids during the process of heating.We have known that TFA will induce arteriosclerosis and other diseases,but the impact on intestinal health is still not clear until now.Hydrogenated soybean oil and yak butter are rich in industrially produced TFA and ruminant TFA,repectively.Therefore,we studied the effects of hydrogenated soybean oil,yak butter and olive oil on the fatty acid composition and immune barrier function in mice intestine,the study will provide evidence for the further study of the effect of TFA on intestinal function.In the present study,male C57-BL mice which were weaned of the third week were used as the study object.The mice were fed freely with the fodder supplemented with edible oil.The different edible oils made the mice divided into four groups:the pressing soya bean oil(control group),the olive oil,the hydrogenated soybean oil and the yak butter.When the mice grew to eight weeks old(adult),the small intestine and colon were taken and the fatty acid composition was determined.Morphological observation was performed by HE staining and the effects of hydrogenated soybean oil and yak butter on intestinal immune barrier function were evaluated by the detection of secretory IgA and IL-8 expression.The results showed that with the increase of mouse age,the body weight growth rate of mice in hydrogenated soybean oil group and yak butter group was faster than that in control group,while there was no significant difference between the four groups of tail length.The content of TFA in the small intestine of the hydrogenated soybean oil group was significantly higher than that of the other three groups.The contents of trans-soleic acid and conjugated linoleic acid in the small intestine of the hydrogenated soybean oil group and the yak butter group were significantly higher than those in the control group.Also there was no significant difference in intestinal histomorphology between the four groups.The dietary intake of fatty acids had no significant effect on the expression of intestinal secretory IgA.The content of IL-8 in the small intestine of the hydrogenated soybean oil group and the yak butter group was significantly decreased,which was mainly due to the TFA induced by dietary intake.In summary,the intake of hydrogenated soybean oil and yak butter significantly changed the intestinal fatty acid composition in mice,and caused a decrease in the content of IL-8 in the small intestine of mice,indicating that the intake of hydrogenated soybean oil and yak butter may influence the function of intestinal immune barrier.