Synthesis Of Fluorescent Noble Metal Nanoclusters And Their Application In The Analysis Of Metal Ions

Noble metal nanoclusters, as a novel fluorescent sensor, has a wide application prospect in optoelectronic devices, catalysis, and analysis. In this paper, several kinds of noble metal nanoparticles were synthesized and had used to detect metal ions. The main contents of this paper are as follows:?1? The first chapter describes the characteristics of noble metal nanomaterials,synthesis of some common noble metal clusters, the principle and development of the fluorescent biosensor probe, and the significance and research contents of this paper.?2? In the second chapter, Ag/Au alloy NCs are protected by glutathione?GSH?.Three ionic including Al³⁺, Cd²⁺ and Pb²⁺ can form core-shell structure with GSH-Au Ag NCs resulting in the fluorescent enhancement of GSH-Au Ag NCs.Different masking agents can be used to detect these three ions, respectively. First,due to F- and Al³⁺ can form a stable complex, F- is added in the system, Al³⁺ can be determined according to the fluorescence change of GSH-Au Ag NCs. Secondly, using sulfosalicylic acid masking Al³⁺ and Cd²⁺, Pb²⁺ can be detection; Finally, Cd²⁺ can be detection by using these two masking agents in the solution. The detection limit of Al³⁺, Cd²⁺ and Pb²⁺ were 4.2×10-8 mol/L, 2.0×10-9 mol/L and 2.4×10-8 mol/L,respectively.?3? In the third chapter, first of all useing the guanidine hydrochloride untwist bovine serum albumin?BSA?, and then using TCEP to restore the disulfide bonds to form a thiol group. After that the obtained single-stranded BSA was used to synthesis gold clusters?d BSA-Au NCs?. The experimental show that Cu²⁺ can quench the fluorescence of gold clusters. Under the optimum conditions, a decent linear relationship for fluorescent detection of Cu²⁺was obtained in the range of 5.0×10-8mol/L 1.0×10-6 mol/L with a 4.5×10-9 mol/L limit of detection.?4? In the fourth chapter, citrate-stabilized Au NPs was prepared by the method of sodium citrate reduction. The prepared gold nanoparticles was characterized by UV-Vis spectrophotometer. Hg²⁺ was detected by the gold nanoparticles-Hg²⁺-cysteine system. The linear range is from 5.0×10-8 mol/L 5.0×10-6 mol/L and the detection limit was 9.2×10-9 mol/L. This method for the detection of Hg²⁺ was rapidly and selectively.