| Anaerobic ammonia oxidation (Anammox) process is a new type of cheap denitrification process, which has high denitrification performance, without adding organic carbon source, no sludge, etc. It gets more and more people's attention.With the in-depth study of this process, researchers found that anammox process exists difficulty in starting and running instability, which seriously restricted the application of the process on a large scale, so adding high performance anammox sludge denitrification and anammox build base have become a kind of mud solution.In this research, the domestication of floc sludge form a sewage treatment plant is to obtain anammox sludge with efficient denitrification ability. Then the anammox activity and observe sludge physiology, which indirect evidence the existence of anammox, were monitored. By the means of PCR-DGGE, metagenomic sequencing directly identified the species of anammox bacteria. On this basis, focusing on preservation characteristics of anammox bacteria and the activity of the recovery process, provide a theoretical basis for the construction of an anaerobic ammonia oxidation species mud base. Research results are as follows:(1) After 122 days of acclimatization Anammox-reactor keeps stabilized and after 129 days of acclimatization anaerobic ammonia oxidation reactor started successfully. Anammox-reactor average removal rates of ammonia nitrogen and nitrite nitrogen were 86.67% and 91.65%, respectively. And the maximumremoval rate of ammonia nitrogen and nitrite nitrogen were 93.34% and 97.91%, respectively. The ratios of average ammonia removal, average nitrite nitrogen removal and the average amount generated nitrate nitrogen were 1:1.41:0.23;(2) The TN(NH4++NO2-) removal capacity of sludge by 0.06 gN/(gVSS · d) increase to 0.24 gN/(gVSS · d),the TN removal rate of sludge from 0.0033 increase to 0.07757. Planctomycetes is about 10% in enriched sludge in this experiment and the planctomycetes contained Candidatus Brocadia and Candidatus Kuenenia genus has anaerobic ammonium oxidation function;(3) Collection of anammox-bacteria play a major role are:preservation preservation time and temperature two factors, in addition to inorganic carbon has a protective effect. Short-term anammox sludge (?30 days) preservation, can be placed in sealed dark at room temperature preservation best; storage period of 60 days to 4? add carbon seal for the best way to preservation preservation temperature in the dark; long-term preservation should be preserved in 4? temperature without adding to carbon deposition can be sealed dark.(4) Anammox sludge deposited 30 days at room temperature and 4? preservation system, after four days of active recovery of its nitrogen capacity lower than 83.3% of the original sludge denitrification performance, after 12 days of active recovery of its nitrogen removal to achieve the original sludge denitrification performance is even higher;Anammox sludge deposited 60 days for a period of 10 days of active recovery,4? system is the best 98.33%,room temperature system and 4?+inorganic carbon system recovery result is 81.25%,the others recovery result is allmost 60%.Anammox sludge deposited 90 days for a period of 10 days of active recovery, preservation system in varying degrees the ability to recover sludge denitrification were:4?+inorganic carbon system> 4? system>-4?+inorganic carbon system>-4? system> room temperature+inorganic carbon system> room temperature system.4?+inorganic carbon system> 4? system recovery result is 59.58%?56.67%?(5) Anammox sludge deposited 110 days for a period of 10 days of active recovery,adding lmg/L,2 mg/L AHLs may cause the total nitrogen removal rate anammox sludge improved 31.54% and 27.53%. Adding 0,1,2mg/L AHLs to recovery Anammox sludge, Its recovery result is 61.88%,64.17% and 49.17%. |
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