Preparation Of Polymer-based Fe₃O₄/mSiO₂ Mesoporous Particles For Controlled Doxorubicin Release

In recent years, magnetic composite nanomaterials are of great interest for researchers from a wide range of biomedical disciplines, which have attracted the attention of scientific research workers by using magnetic nanomaterials as a drug carrier has become a hot spot of research. Nano-engineering the magnetic nanoparticles and polymer materials into a single entity would not only improve the stability of magnetic nanoparticles, but also generate biological compatibility and environmental responsiveness. Therefore these materials have widely application prospects in targeted drug delivery field. Fe₃O₄ /mSi O₂ nanoparticles were prepared by solvent hot method with the block copolymer and hexadecyl trimethyl ammonium bromide?CTAB? as the templates,and TEOS as the silicon source at different experimental conditions, The properties and morphology of naoparticles were analysized. This Fe₃O₄ /mSiO₂ were used to synthesis polymer-based magnetic mesoporous nanomaterials with polymer. Thus the nanoparticles are expected to application in drug delivery and release. The subject of the work is summarized as follows:?1?Different size, better dispersion polymer modified Fe₃O₄ nanoparticles were synthesized by solvent hot method, Fe₃O₄ /mSiO₂ were synthesized by sol gel method with block copolymer and surfactant cetyl trimethyl ammonium bromide?CTAB? as porous template. We also studied the influence of different conditions on the morphology and size of Fe₃O₄ /mSiO₂. Drug delivery and curative effect can improved by porous structure. In this nanoparticles, magnetic not only plays a targeting role, but also protects the normal cells at the same time. The experimental results show that better dispersion Fe₃O₄ particles will get when use PAA- b- PMAPEG modified, reaction 2 h, which have smaller particle size about 20 nm. When the reation condition is under 35 ?, with the amount of 2 mL TEOS,we could gain the size is about 80 nm magnetic Fe₃O₄ /mSiO₂ nanoparticles.This lay a foundation for further modification and application.?2?Modified vinyl functional of porous Fe₃O₄ /mSiO₂ to prepare Fe₃O₄ /mSiO₂ / MPS. We synthesized it with block copolymer poly(butyl acrylate copolymer- acrylic?P?IBA- co- AA?? to prepare Fe₃O₄ /mSiO₂ / P?IBA- co- AA? by reversible addition fragmentation chain transfer radical?RAFT? polymerization. The novel magnetic composite nanomaterials were subsequently characterized by FT-IR spectroscopy. The drug delivery behaviors of the micelles embedding anti-cancer drug Doxorubicin?DOX? in a simulated human body environment were studied. The in vivo antitumor activity was evaluated in liver cancer SMCC7211 cells. MTT assay was used to evaluate cytotoxicity of the magnetic composite nanomaterials. The results showed that the Fe₃O₄ /mSiO₂ / P?IBA- co- AA? was one of novel and ideal biomaterials as DOX nano-carrier, drug-loading particles can effectively release DOX drugs to inhibit the proliferation of cancer cells.?3?Modified vinyl functional of porous Fe₃O₄ /mSiO₂ to prepare Fe₃O₄ /mSiO₂ / MPS, then adopts it wih block copolymer poly?methyl acryloyl oxygen quinoline- copolymerization of acrylic?- block-polyethylene glycol methyl methacrylate?P?MAQ- co- AA?- b- PMAPEG? to prepare Fe₃O₄ /mSiO₂ / P?MAQ- co- AA?- b- PMAPEG by reversible addition fragmentation chain transfer radical?RAFT? polymerization. The composites material have magnetic responsiveness, light responsive and pH responsive, these specific properties make the material become a novel materials of magnetic guide, fluorescent tracer and pH responsive drug carrier in drug control and release. The drug delivery behaviors of the micelles embedding anti-cancer drug Doxorubicin?DOX? in a simulated human body environment were studied. The in vivo antitumor activity was evaluated in liver cancer SMCC7211 cells. MTT assay was used to evaluate cytotoxicity of the magnetic composite nanomaterials. The results showed that the Fe₃O₄ /mSiO₂ / P?MAQ- co- AA?- b- PMAPEG drug-loading particles under simulated conditions of human physiological reveal the stability of the drug release behavior, and enhance the antitumor activity of the drug while significantly reduce its side effects. This material has potential application prospect as an anti-cancer drug targeting delivery carrier.