| In today’s society, all kinds of diseases can be effectively controlled by the contribution of antibiotics, Because it plays a strong role in the sterilization and inhibition of bacterial growth, so it is widely used in animal, human and poultry diseases control. In fact, the scientific use of antibiotics can be effective in the treatment of many diseases, but once the high doses or abuse even to the extent, which will cause irreparable consequences for health.In order to regulate the use of antibiotics, many countries have introduced the corresponding policies and regulations. But in the field of medicine and food, because the rational use of standardized mechanism has not yet formed expressly to supervise the abuse of antibiotics, the situation is still very serious. If we can achieve ways to monitore the drugs of antibiotics, which can effectively regulate the use of Medicine. Therefore, if we can realize the rapid and simple detection, it is particularly important. At present, there are some methods for the detection of antibiotics, but they are limited by their own defects. Now, the chromatographic analysis method is more commonly used in the test method of antibiotics. However, there are many technical difficulties in the analysis of qualitative analysis in multicomponent mixtures by chromatography, so having no ability to analyze complex compounds. Now, Raman detection technology is becoming more and more mature, and Surface enhanced Raman spectroscopy technology can realize the single molecule detection, qualitative and quantitative analysis of a small sample, and has high accuracy and sensitivity, which is expected to realize the detection of antibiotics.Raman scattering is an important and common scattering phenomenon in optics, which is produced by the vibration of molecules and lattices. Raman spectroscopy is based on Raman scattering effect, it has the advantages that other detection techniques can not be compared, for example:no need for special markers of the sample, fast, and can be real-time online detection, etc. Because the Raman scattering signal is weak. Therefore, in this paper, surface enhanced Raman spectroscopy was applied to the chloramphenicol rapid detection, overcoming the characteristics of Raman scattering weak signal, commonly used chloramphenicol was detected by SERS. In this paper, the basic principle and the surface enhanced Raman scattering as the main content,according to the following two aspects:First, this thesis has done a brief introduction to properties and use of hormone and chloramphenicol, introducting the general knowledge of Raman spectroscopy, describing the surface enhanced Raman spectroscopy and briefly outlines the characteristics of ZnO thin films.Then, three kinds of SERS active substrates were introduced, and comparing and analying the enhancement effects of the three substrates. Through experiments, we found a novel method and simple preparation of silver sol -Zinc Oxide- silicon composite substrate which has better stability. Three kinds of substrate were prepared, respectively, silver-Zinc Oxide-silicon substrate, silver colloidal- Zinc Oxide - silicon substrate, silver sol - silicon substrate, comparing pulling speed, photocatalysis and photocatalytic reduction of silver and water bath heating generating silver colloid. The preliminary findings of the stability of three kinds of substrates are good, can obtain better effect of enhanced Raman spectra of chloramphenicol. Compared to traditional silver plastic substrate,the silver colloidal -oxide zinc -silicon enhancement effect is better, the operation is simple; followed by silver colloidal- silicon and silver -oxide zinc - silicon, compared to the latter, the former operation is more convenient; considering that silver colloidal-oxide zinc-silicon substrate of preparing cost is low, enhanced signal is strong, the operation is simple, the most suitable for the detection of chloramphenicol. |
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